Mesenchymal stem cells prolong composite tissue allotransplant survival in a swine model

Yur Ren Kuo, Shigeru Goto, Hsiang Shun Shih, Feng Sheng Wang, Chien Chih Lin, Chun Ting Wang, Eng Yen Huang, Chao Long Chen, Fu Chan Wei, Xin Xiao Zheng, W P Andrew Lee

Research output: Contribution to journalArticle

Abstract

BACKGROUND.: This study investigated whether mesenchymal stem cells (MSCs) combined with bone marrow transplantation (BMT), irradiation, or short-term immunosuppressant therapy could prolong composite tissue allotransplant survival in a swine hind-limb model. METHODS.: Heterotopic hind-limb transplantation was performed in outbred miniature swine. Group I (n=5) was the untreated control. Group II (n=3) received MSCs alone (given on days -1, +3, +7, +14, +21). Group III (n=6) received cyclosporine A (CsA days 0 to +28). Group IV (n=4) received preconditioning irradiation (day -1), BMT (day +1), and CsA (days 0 to +28). Group V (n=5) received irradiation (day -1), BMT (day +1), CsA (days 0 to +28), and MSCs (days +1, +7,+14). The expression and localization of CD4/CD25 T cells and MSCs were assessed using flow cytometry and immunohistochemistry. RESULTS.: The allografts survival with MSCs alone revealed a significant prolongation, when compared with the controls (P=0.02). Allografts with CsA treatment exhibited delayed rejection. Irradiation and BMT-CsA treatment revealed no significant allograft survival benefit when compared with the CsA treatment group, but graft-versus-host disease (GVHD) was evident. However, combination of MSCs-BMT-CsA treatment demonstrated significant prolongation of allograft survival (>200 days, P

Original languageEnglish (US)
Pages (from-to)1769-1777
Number of pages9
JournalTransplantation
Volume87
Issue number12
DOIs
StatePublished - Jun 27 2009
Externally publishedYes

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Tissue Survival
Mesenchymal Stromal Cells
Bone Marrow Transplantation
Swine
Allografts
Extremities
Therapeutics
Miniature Swine
Graft vs Host Disease
Immunosuppressive Agents
Cyclosporine
Flow Cytometry
Transplantation
Immunohistochemistry
T-Lymphocytes

Keywords

  • Composite tissue allotransplant
  • Mesenchymal stem cells

ASJC Scopus subject areas

  • Transplantation

Cite this

Kuo, Y. R., Goto, S., Shih, H. S., Wang, F. S., Lin, C. C., Wang, C. T., ... Lee, W. P. A. (2009). Mesenchymal stem cells prolong composite tissue allotransplant survival in a swine model. Transplantation, 87(12), 1769-1777. https://doi.org/10.1097/TP.0b013e3181a664f1

Mesenchymal stem cells prolong composite tissue allotransplant survival in a swine model. / Kuo, Yur Ren; Goto, Shigeru; Shih, Hsiang Shun; Wang, Feng Sheng; Lin, Chien Chih; Wang, Chun Ting; Huang, Eng Yen; Chen, Chao Long; Wei, Fu Chan; Zheng, Xin Xiao; Lee, W P Andrew.

In: Transplantation, Vol. 87, No. 12, 27.06.2009, p. 1769-1777.

Research output: Contribution to journalArticle

Kuo, YR, Goto, S, Shih, HS, Wang, FS, Lin, CC, Wang, CT, Huang, EY, Chen, CL, Wei, FC, Zheng, XX & Lee, WPA 2009, 'Mesenchymal stem cells prolong composite tissue allotransplant survival in a swine model', Transplantation, vol. 87, no. 12, pp. 1769-1777. https://doi.org/10.1097/TP.0b013e3181a664f1
Kuo, Yur Ren ; Goto, Shigeru ; Shih, Hsiang Shun ; Wang, Feng Sheng ; Lin, Chien Chih ; Wang, Chun Ting ; Huang, Eng Yen ; Chen, Chao Long ; Wei, Fu Chan ; Zheng, Xin Xiao ; Lee, W P Andrew. / Mesenchymal stem cells prolong composite tissue allotransplant survival in a swine model. In: Transplantation. 2009 ; Vol. 87, No. 12. pp. 1769-1777.
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AU - Kuo, Yur Ren

AU - Goto, Shigeru

AU - Shih, Hsiang Shun

AU - Wang, Feng Sheng

AU - Lin, Chien Chih

AU - Wang, Chun Ting

AU - Huang, Eng Yen

AU - Chen, Chao Long

AU - Wei, Fu Chan

AU - Zheng, Xin Xiao

AU - Lee, W P Andrew

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