Merkel cell carcinoma arising in inguinal lymph node in a patient with von Willebrand disease after multiple blood transfusions

Renzo Boldorini, Sara Allegrini, Mauro Tognon, Umberto Miglio, Davide Rossi, Michael Pawlita, Raphael P Viscidi

Research output: Contribution to journalArticle

Abstract

Merkel cell carcinoma (MCC) is an uncommon neuroendocrine tumour of the skin; rare cases have been reported within the lymph nodes without a primary site. The detection of Merkel cell polyomavirus (MCPyV) DNA, integrated within the genome of MCC, suggests its role for the onset of this tumour. We report a case of MCC in an inguinal lymph node of a patient with Von Willebrand disease (VWD), who underwent multiple blood transfusions following haemorrhoidectomy. The diagnosis was performed on the bases of morphology and immunohistochemistry; genomic sequences of LT and VP1 regions of MCPyV were amplified from MCC using a quantitative polymerase chain reaction (qPCR) assay. High levels of MCPyV antibodies were detected in the patient's serum by ELISA method. We discuss the role of MCPyV in the development of this tumour, the use of viral DNA detection for confirming the diagnosis of MCC in unusual sites and the possibility of MCPyV transmission from blood donors.

Original languageEnglish (US)
Pages (from-to)73-75
Number of pages3
JournalJournal of Clinical Virology
Volume60
Issue number1
DOIs
StatePublished - 2014

Fingerprint

Merkel cell polyomavirus
Merkel Cell Carcinoma
von Willebrand Diseases
Groin
Blood Transfusion
Lymph Nodes
Hemorrhoidectomy
Neuroendocrine Tumors
Viral DNA
Blood Donors
Neoplasms
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry
Genome
Polymerase Chain Reaction
Skin
Antibodies
DNA
Serum

Keywords

  • Lymph node
  • Merkel cell carcinoma (MCC)
  • Merkel cell polyomavirus (MCPyV)
  • Von Willebrand disease

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases
  • Medicine(all)

Cite this

Merkel cell carcinoma arising in inguinal lymph node in a patient with von Willebrand disease after multiple blood transfusions. / Boldorini, Renzo; Allegrini, Sara; Tognon, Mauro; Miglio, Umberto; Rossi, Davide; Pawlita, Michael; Viscidi, Raphael P.

In: Journal of Clinical Virology, Vol. 60, No. 1, 2014, p. 73-75.

Research output: Contribution to journalArticle

Boldorini, Renzo ; Allegrini, Sara ; Tognon, Mauro ; Miglio, Umberto ; Rossi, Davide ; Pawlita, Michael ; Viscidi, Raphael P. / Merkel cell carcinoma arising in inguinal lymph node in a patient with von Willebrand disease after multiple blood transfusions. In: Journal of Clinical Virology. 2014 ; Vol. 60, No. 1. pp. 73-75.
@article{e2df6ca7fe774559ad1c79420c8aa9bb,
title = "Merkel cell carcinoma arising in inguinal lymph node in a patient with von Willebrand disease after multiple blood transfusions",
abstract = "Merkel cell carcinoma (MCC) is an uncommon neuroendocrine tumour of the skin; rare cases have been reported within the lymph nodes without a primary site. The detection of Merkel cell polyomavirus (MCPyV) DNA, integrated within the genome of MCC, suggests its role for the onset of this tumour. We report a case of MCC in an inguinal lymph node of a patient with Von Willebrand disease (VWD), who underwent multiple blood transfusions following haemorrhoidectomy. The diagnosis was performed on the bases of morphology and immunohistochemistry; genomic sequences of LT and VP1 regions of MCPyV were amplified from MCC using a quantitative polymerase chain reaction (qPCR) assay. High levels of MCPyV antibodies were detected in the patient's serum by ELISA method. We discuss the role of MCPyV in the development of this tumour, the use of viral DNA detection for confirming the diagnosis of MCC in unusual sites and the possibility of MCPyV transmission from blood donors.",
keywords = "Lymph node, Merkel cell carcinoma (MCC), Merkel cell polyomavirus (MCPyV), Von Willebrand disease",
author = "Renzo Boldorini and Sara Allegrini and Mauro Tognon and Umberto Miglio and Davide Rossi and Michael Pawlita and Viscidi, {Raphael P}",
year = "2014",
doi = "10.1016/j.jcv.2014.02.007",
language = "English (US)",
volume = "60",
pages = "73--75",
journal = "Journal of Clinical Virology",
issn = "1386-6532",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Merkel cell carcinoma arising in inguinal lymph node in a patient with von Willebrand disease after multiple blood transfusions

AU - Boldorini, Renzo

AU - Allegrini, Sara

AU - Tognon, Mauro

AU - Miglio, Umberto

AU - Rossi, Davide

AU - Pawlita, Michael

AU - Viscidi, Raphael P

PY - 2014

Y1 - 2014

N2 - Merkel cell carcinoma (MCC) is an uncommon neuroendocrine tumour of the skin; rare cases have been reported within the lymph nodes without a primary site. The detection of Merkel cell polyomavirus (MCPyV) DNA, integrated within the genome of MCC, suggests its role for the onset of this tumour. We report a case of MCC in an inguinal lymph node of a patient with Von Willebrand disease (VWD), who underwent multiple blood transfusions following haemorrhoidectomy. The diagnosis was performed on the bases of morphology and immunohistochemistry; genomic sequences of LT and VP1 regions of MCPyV were amplified from MCC using a quantitative polymerase chain reaction (qPCR) assay. High levels of MCPyV antibodies were detected in the patient's serum by ELISA method. We discuss the role of MCPyV in the development of this tumour, the use of viral DNA detection for confirming the diagnosis of MCC in unusual sites and the possibility of MCPyV transmission from blood donors.

AB - Merkel cell carcinoma (MCC) is an uncommon neuroendocrine tumour of the skin; rare cases have been reported within the lymph nodes without a primary site. The detection of Merkel cell polyomavirus (MCPyV) DNA, integrated within the genome of MCC, suggests its role for the onset of this tumour. We report a case of MCC in an inguinal lymph node of a patient with Von Willebrand disease (VWD), who underwent multiple blood transfusions following haemorrhoidectomy. The diagnosis was performed on the bases of morphology and immunohistochemistry; genomic sequences of LT and VP1 regions of MCPyV were amplified from MCC using a quantitative polymerase chain reaction (qPCR) assay. High levels of MCPyV antibodies were detected in the patient's serum by ELISA method. We discuss the role of MCPyV in the development of this tumour, the use of viral DNA detection for confirming the diagnosis of MCC in unusual sites and the possibility of MCPyV transmission from blood donors.

KW - Lymph node

KW - Merkel cell carcinoma (MCC)

KW - Merkel cell polyomavirus (MCPyV)

KW - Von Willebrand disease

UR - http://www.scopus.com/inward/record.url?scp=84898925807&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84898925807&partnerID=8YFLogxK

U2 - 10.1016/j.jcv.2014.02.007

DO - 10.1016/j.jcv.2014.02.007

M3 - Article

C2 - 24630954

AN - SCOPUS:84898925807

VL - 60

SP - 73

EP - 75

JO - Journal of Clinical Virology

JF - Journal of Clinical Virology

SN - 1386-6532

IS - 1

ER -