Meningothelial cells react to elevated pressure and oxidative stress

Xiaorong Xin, Bin Fan, Josef Flammer, Neil R Miller, Gregor P. Jaggi, Hanspeter E. Killer, Peter Meyer, Albert Neutzner

Research output: Contribution to journalArticle

Abstract

Background: Meningothelial cells (MECs) are the cellular components of the meninges enveloping the brain. Although MECs are not fully understood, several functions of these cells have been described. The presence of desmosomes and tight junctions between MECs hints towards a barrier function protecting the brain. In addition, MECs perform endocytosis and, by the secretion of cytokines, are involved in immunological processes in the brain. However, little is known about the influence of pathological conditions on MEC function; e.g., during diseases associated with elevated intracranial pressure, hypoxia or increased oxidative stress. Methods: We studied the effect of elevated pressure, hypoxia, and oxidative stress on immortalized human as well as primary porcine MECs. We used MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) bioreduction assays to assess the proliferation of MECs in response to treatment and compared to untreated control cells. To assess endocytotic activity, the uptake of fluorescently labeled latex beads was analyzed by fluorescence microscopy. Results: We found that exposure of MECs to elevated pressure caused significant cellular proliferation and a dramatic decrease in endocytotic activity. In addition, mild oxidative stress severely inhibited endocytosis. Conclusion: Elevated pressure and oxidative stress impact MEC physiology and might therefore influence the microenvironment of the subarachnoid space and thus the cerebrospinal fluid within this compartment with potential negative impact on neuronal function.

Original languageEnglish (US)
Article numbere20142
JournalPLoS One
Volume6
Issue number5
DOIs
StatePublished - 2011

Fingerprint

Oxidative stress
Oxidative Stress
oxidative stress
Pressure
Brain
cells
Cerebrospinal fluid
Fluorescence microscopy
Latex
Physiology
Endocytosis
endocytosis
brain
Assays
hypoxia
cell proliferation
Cell Proliferation
Cytokines
Cell Physiological Phenomena
Meninges

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Xin, X., Fan, B., Flammer, J., Miller, N. R., Jaggi, G. P., Killer, H. E., ... Neutzner, A. (2011). Meningothelial cells react to elevated pressure and oxidative stress. PLoS One, 6(5), [e20142]. https://doi.org/10.1371/journal.pone.0020142

Meningothelial cells react to elevated pressure and oxidative stress. / Xin, Xiaorong; Fan, Bin; Flammer, Josef; Miller, Neil R; Jaggi, Gregor P.; Killer, Hanspeter E.; Meyer, Peter; Neutzner, Albert.

In: PLoS One, Vol. 6, No. 5, e20142, 2011.

Research output: Contribution to journalArticle

Xin, X, Fan, B, Flammer, J, Miller, NR, Jaggi, GP, Killer, HE, Meyer, P & Neutzner, A 2011, 'Meningothelial cells react to elevated pressure and oxidative stress', PLoS One, vol. 6, no. 5, e20142. https://doi.org/10.1371/journal.pone.0020142
Xin, Xiaorong ; Fan, Bin ; Flammer, Josef ; Miller, Neil R ; Jaggi, Gregor P. ; Killer, Hanspeter E. ; Meyer, Peter ; Neutzner, Albert. / Meningothelial cells react to elevated pressure and oxidative stress. In: PLoS One. 2011 ; Vol. 6, No. 5.
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