Membrane trafficking of collecting duct water channel protein AQP2 regulated by Akt/AS160

Hyun Jun Jung, Tae Hwan Kwon

Research output: Contribution to journalReview article

Abstract

Akt (protein kinase B (PKB)) is a serine/threonine kinase that acts in the phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway. The PI3K/Akt signaling pathway, triggered by growth factors and hormones including vasopressin, is an important pathway that is widely involved in cellular mechanisms regulating transcription, translation, cell growth and death, cell proliferation, migration, and cell cycles. In particular, Akt and Akt substrate protein of 160 kDa (AS160) are likely to participate in the trafficking of aquaporin-2 (AQP2) in the kidney collecting duct. In this study, we demonstrated that 1) small interfering RNA (siRNA)-mediated gene silencing of Akt1 significantly decreased Akt1 and phospho-AS160 protein expression; and 2) confocal laser scanning microscopy of AQP2 in mouse cortical collecting duct cells (M-1 cells) revealed AS160 knockdown by siRNA increased AQP2 expression in the plasma membrane compared with controls, despite the absence of dDAVP stimulation. Thus, the results suggest that PI3K/Akt pathways could play a role in AQP2 trafficking via the AS160 protein.

Original languageEnglish (US)
Pages (from-to)59-65
Number of pages7
JournalElectrolyte and Blood Pressure
Volume8
Issue number2
DOIs
StatePublished - Dec 1 2010
Externally publishedYes

Fingerprint

Aquaporin 2
Aquaporins
Phosphatidylinositol 3-Kinase
Membranes
Small Interfering RNA
Collecting Kidney Tubules
Proto-Oncogene Proteins c-akt
Proteins
Protein-Serine-Threonine Kinases
Gene Silencing
Vasopressins
Confocal Microscopy
Growth Hormone
Cell Movement
Intercellular Signaling Peptides and Proteins
Cell Cycle
Cell Death
Cell Proliferation
Cell Membrane
Growth

Keywords

  • Aquaporin 2
  • Protein kinase b
  • Protein transport
  • Rab gtp-binding proteins
  • Tbc1d4 protein

ASJC Scopus subject areas

  • Internal Medicine
  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Membrane trafficking of collecting duct water channel protein AQP2 regulated by Akt/AS160. / Jung, Hyun Jun; Kwon, Tae Hwan.

In: Electrolyte and Blood Pressure, Vol. 8, No. 2, 01.12.2010, p. 59-65.

Research output: Contribution to journalReview article

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AB - Akt (protein kinase B (PKB)) is a serine/threonine kinase that acts in the phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway. The PI3K/Akt signaling pathway, triggered by growth factors and hormones including vasopressin, is an important pathway that is widely involved in cellular mechanisms regulating transcription, translation, cell growth and death, cell proliferation, migration, and cell cycles. In particular, Akt and Akt substrate protein of 160 kDa (AS160) are likely to participate in the trafficking of aquaporin-2 (AQP2) in the kidney collecting duct. In this study, we demonstrated that 1) small interfering RNA (siRNA)-mediated gene silencing of Akt1 significantly decreased Akt1 and phospho-AS160 protein expression; and 2) confocal laser scanning microscopy of AQP2 in mouse cortical collecting duct cells (M-1 cells) revealed AS160 knockdown by siRNA increased AQP2 expression in the plasma membrane compared with controls, despite the absence of dDAVP stimulation. Thus, the results suggest that PI3K/Akt pathways could play a role in AQP2 trafficking via the AS160 protein.

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