Membrane potential depolarization as a triggering mechanism for Vpu-mediated HIV-1 release

Kate Hsu, Jin Han, Krekwit Shinlapawittayatorn, Isabelle Deschenes, Eduardo Marbán

Research output: Contribution to journalArticle

Abstract

Vpu, a component unique to HIV-1, greatly enhances the efficiency of viral particle release by unclear mechanisms. This Vpu function is intrinsically linked to its channel-like structure, which enables it to interfere with homologous transmembrane structures in infected cells. Because Vpu interacts destructively with host background K+ channels that set the cell resting potential, we hypothesized that Vpu might trigger viral release by destabilizing the electric field across a budding membrane. Here, we found that the efficiency of Vpu-mediated viral release is inversely correlated with membrane potential polarization. By inhibiting the background K+ currents, Vpu dissipates the voltage constraint on viral particle discharge. As a proof of concept, we show that HIV-1 release can be accelerated by externally imposed depolarization alone. Our findings identify the trigger of Vpu-mediated release as a manifestation of the general principle of depolarization-stimulated exocytosis.

Original languageEnglish (US)
Pages (from-to)1718-1725
Number of pages8
JournalBiophysical Journal
Volume99
Issue number6
DOIs
StatePublished - 2010
Externally publishedYes

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Virion
Membrane Potentials
HIV-1
Exocytosis
Membranes

ASJC Scopus subject areas

  • Biophysics

Cite this

Hsu, K., Han, J., Shinlapawittayatorn, K., Deschenes, I., & Marbán, E. (2010). Membrane potential depolarization as a triggering mechanism for Vpu-mediated HIV-1 release. Biophysical Journal, 99(6), 1718-1725. https://doi.org/10.1016/j.bpj.2010.07.027

Membrane potential depolarization as a triggering mechanism for Vpu-mediated HIV-1 release. / Hsu, Kate; Han, Jin; Shinlapawittayatorn, Krekwit; Deschenes, Isabelle; Marbán, Eduardo.

In: Biophysical Journal, Vol. 99, No. 6, 2010, p. 1718-1725.

Research output: Contribution to journalArticle

Hsu, K, Han, J, Shinlapawittayatorn, K, Deschenes, I & Marbán, E 2010, 'Membrane potential depolarization as a triggering mechanism for Vpu-mediated HIV-1 release', Biophysical Journal, vol. 99, no. 6, pp. 1718-1725. https://doi.org/10.1016/j.bpj.2010.07.027
Hsu, Kate ; Han, Jin ; Shinlapawittayatorn, Krekwit ; Deschenes, Isabelle ; Marbán, Eduardo. / Membrane potential depolarization as a triggering mechanism for Vpu-mediated HIV-1 release. In: Biophysical Journal. 2010 ; Vol. 99, No. 6. pp. 1718-1725.
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