Membrane microextension: A possible mechanism for establishing molecular contact in electrofusion

Subrata Biswas, Sujoy K. Guha

Research output: Contribution to journalArticle

Abstract

True cell membrane contact is an essential condition for electro-pulsed cell fusion, but initial morphological perturbation leading to true contact is still not clear. Dielectrophoresis mediated compression and fusogenic pulse induced compaction of cells led to rapid merger of tight membranes, and deprived direct microscopic view of surface membrane perturbation. Freely suspending cells with large and different cell-cell gaps may proceed to electrofusion with perturbed membrane and initiates fusion events at different time. These pulsed exposed cells can be used for capturing changes in the membrane surface and early electrofusion events. Early stage of fusion of freely suspended intact human erythrocytes exposed to single exponential decay pulse was studied by scanning electron microscopy (SEM). Field pulse induces small membrane bumps. Interaction of bumps on adjacent membranes lead to true membrane contact and form bridges between the membranes as microextension, combining both membranes into a topologically single structure. Some fusion products showed expanded fusion zones, which suggest indication of open lumen at contact area. Copyright (C) 1999 Elsevier Science S.A.

Original languageEnglish (US)
Pages (from-to)435-440
Number of pages6
JournalBioelectrochemistry and Bioenergetics
Volume48
Issue number2
DOIs
StatePublished - May 1999
Externally publishedYes

Fingerprint

membranes
Membranes
Fusion reactions
fusion
cells
Compaction
pulses
Membrane Fusion
Cell Fusion
perturbation
lumens
erythrocytes
Electron Scanning Microscopy
Cell membranes
Electrophoresis
Erythrocytes
Cell Membrane
indication
Scanning electron microscopy
scanning electron microscopy

Keywords

  • Electrofusion
  • Membrane bump
  • Membrane contact
  • Membrane microextension
  • Morphological perturbation

ASJC Scopus subject areas

  • Biochemistry

Cite this

Membrane microextension : A possible mechanism for establishing molecular contact in electrofusion. / Biswas, Subrata; Guha, Sujoy K.

In: Bioelectrochemistry and Bioenergetics, Vol. 48, No. 2, 05.1999, p. 435-440.

Research output: Contribution to journalArticle

@article{037cdf325dcd40658675b4d9c8a1f0e8,
title = "Membrane microextension: A possible mechanism for establishing molecular contact in electrofusion",
abstract = "True cell membrane contact is an essential condition for electro-pulsed cell fusion, but initial morphological perturbation leading to true contact is still not clear. Dielectrophoresis mediated compression and fusogenic pulse induced compaction of cells led to rapid merger of tight membranes, and deprived direct microscopic view of surface membrane perturbation. Freely suspending cells with large and different cell-cell gaps may proceed to electrofusion with perturbed membrane and initiates fusion events at different time. These pulsed exposed cells can be used for capturing changes in the membrane surface and early electrofusion events. Early stage of fusion of freely suspended intact human erythrocytes exposed to single exponential decay pulse was studied by scanning electron microscopy (SEM). Field pulse induces small membrane bumps. Interaction of bumps on adjacent membranes lead to true membrane contact and form bridges between the membranes as microextension, combining both membranes into a topologically single structure. Some fusion products showed expanded fusion zones, which suggest indication of open lumen at contact area. Copyright (C) 1999 Elsevier Science S.A.",
keywords = "Electrofusion, Membrane bump, Membrane contact, Membrane microextension, Morphological perturbation",
author = "Subrata Biswas and Guha, {Sujoy K.}",
year = "1999",
month = "5",
doi = "10.1016/S0302-4598(99)00043-4",
language = "English (US)",
volume = "48",
pages = "435--440",
journal = "Bioelectrochemistry",
issn = "1567-5394",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Membrane microextension

T2 - A possible mechanism for establishing molecular contact in electrofusion

AU - Biswas, Subrata

AU - Guha, Sujoy K.

PY - 1999/5

Y1 - 1999/5

N2 - True cell membrane contact is an essential condition for electro-pulsed cell fusion, but initial morphological perturbation leading to true contact is still not clear. Dielectrophoresis mediated compression and fusogenic pulse induced compaction of cells led to rapid merger of tight membranes, and deprived direct microscopic view of surface membrane perturbation. Freely suspending cells with large and different cell-cell gaps may proceed to electrofusion with perturbed membrane and initiates fusion events at different time. These pulsed exposed cells can be used for capturing changes in the membrane surface and early electrofusion events. Early stage of fusion of freely suspended intact human erythrocytes exposed to single exponential decay pulse was studied by scanning electron microscopy (SEM). Field pulse induces small membrane bumps. Interaction of bumps on adjacent membranes lead to true membrane contact and form bridges between the membranes as microextension, combining both membranes into a topologically single structure. Some fusion products showed expanded fusion zones, which suggest indication of open lumen at contact area. Copyright (C) 1999 Elsevier Science S.A.

AB - True cell membrane contact is an essential condition for electro-pulsed cell fusion, but initial morphological perturbation leading to true contact is still not clear. Dielectrophoresis mediated compression and fusogenic pulse induced compaction of cells led to rapid merger of tight membranes, and deprived direct microscopic view of surface membrane perturbation. Freely suspending cells with large and different cell-cell gaps may proceed to electrofusion with perturbed membrane and initiates fusion events at different time. These pulsed exposed cells can be used for capturing changes in the membrane surface and early electrofusion events. Early stage of fusion of freely suspended intact human erythrocytes exposed to single exponential decay pulse was studied by scanning electron microscopy (SEM). Field pulse induces small membrane bumps. Interaction of bumps on adjacent membranes lead to true membrane contact and form bridges between the membranes as microextension, combining both membranes into a topologically single structure. Some fusion products showed expanded fusion zones, which suggest indication of open lumen at contact area. Copyright (C) 1999 Elsevier Science S.A.

KW - Electrofusion

KW - Membrane bump

KW - Membrane contact

KW - Membrane microextension

KW - Morphological perturbation

UR - http://www.scopus.com/inward/record.url?scp=0032962008&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032962008&partnerID=8YFLogxK

U2 - 10.1016/S0302-4598(99)00043-4

DO - 10.1016/S0302-4598(99)00043-4

M3 - Article

C2 - 10379565

AN - SCOPUS:0032962008

VL - 48

SP - 435

EP - 440

JO - Bioelectrochemistry

JF - Bioelectrochemistry

SN - 1567-5394

IS - 2

ER -