Membrane-membrane and membrane-substrate adhesion during dissection of gap junctions with the atomic-force microscope

Jan H. Hoh, Jean Paul Revel, Paul K. Hansma

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The gap junction is a specialized region of the plasma membrane that consists of an array of cell-to-cell ion channels. These channels form where the membranes from two cells come together, and the gap junction is therefore composed of two lipid bilayers. The atomic force microscope (AFM) can be used to dissect the gap junction, removing one membrane and exposing the extracellular domains of the second. The force required to dissect the membrane, near 10-8 N vertical force for gap junctions adsorbed to mica, provides a measure of the strength of the interaction between the two membranes. Since a single membrane is left in contact with the mica, this interaction must be stronger than the membrane-membrane interaction. Non-junctional membrane attached to the gap junctions is easily removed with the AFM tip while the gap junction membrane remains attached to the mica, providing evidence that the interaction with the mica is mainly mediated by protein-mica interactions. Consistent with this hypothesis is the observation that material trapped under the membrane sometimes results in pieces of membrane above the material being pulled out during dissection. These results lay the foundation for examining the molecular details of the basis for membrane- membrane and membrane-substrate adhesion.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherPubl by Int Soc for Optical Engineering
Pages212-215
Number of pages4
Volume1639
ISBN (Print)0819407852
StatePublished - 1992
Externally publishedYes
EventScanning Probe Microscopies - Los Angeles, CA, USA
Duration: Jan 20 1992Jan 22 1992

Other

OtherScanning Probe Microscopies
CityLos Angeles, CA, USA
Period1/20/921/22/92

Fingerprint

Dissection
dissection
Microscopes
adhesion
Adhesion
microscopes
membranes
Membranes
Substrates
Mica
mica
interactions
cells
Lipid bilayers
Cell membranes

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Hoh, J. H., Revel, J. P., & Hansma, P. K. (1992). Membrane-membrane and membrane-substrate adhesion during dissection of gap junctions with the atomic-force microscope. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 1639, pp. 212-215). Publ by Int Soc for Optical Engineering.

Membrane-membrane and membrane-substrate adhesion during dissection of gap junctions with the atomic-force microscope. / Hoh, Jan H.; Revel, Jean Paul; Hansma, Paul K.

Proceedings of SPIE - The International Society for Optical Engineering. Vol. 1639 Publ by Int Soc for Optical Engineering, 1992. p. 212-215.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Hoh, JH, Revel, JP & Hansma, PK 1992, Membrane-membrane and membrane-substrate adhesion during dissection of gap junctions with the atomic-force microscope. in Proceedings of SPIE - The International Society for Optical Engineering. vol. 1639, Publ by Int Soc for Optical Engineering, pp. 212-215, Scanning Probe Microscopies, Los Angeles, CA, USA, 1/20/92.
Hoh JH, Revel JP, Hansma PK. Membrane-membrane and membrane-substrate adhesion during dissection of gap junctions with the atomic-force microscope. In Proceedings of SPIE - The International Society for Optical Engineering. Vol. 1639. Publ by Int Soc for Optical Engineering. 1992. p. 212-215
Hoh, Jan H. ; Revel, Jean Paul ; Hansma, Paul K. / Membrane-membrane and membrane-substrate adhesion during dissection of gap junctions with the atomic-force microscope. Proceedings of SPIE - The International Society for Optical Engineering. Vol. 1639 Publ by Int Soc for Optical Engineering, 1992. pp. 212-215
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