Melanopsin signalling in mammalian iris and retina

T. Xue, M. T H Do, A. Riccio, Zheng Jiang, J. Hsieh, H. C. Wang, Shannath L Merbs, D. S. Welsbie, T. Yoshioka, P. Weissgerber, S. Stolz, V. Flockerzi, M. Freichel, M. I. Simon, D. E. Clapham, King-Wai Yau

Research output: Contribution to journalArticle

Abstract

Non-mammalian vertebrates have an intrinsically photosensitive iris and thus a local pupillary light reflex (PLR). In contrast, it is thought that the PLR in mammals generally requires neuronal circuitry connecting the eye and the brain. Here we report that an intrinsic component of the PLR is in fact widespread in nocturnal and crepuscular mammals. In mouse, this intrinsic PLR requires the visual pigment melanopsin; it also requires PLCβ4, a vertebrate homologue of the Drosophila NorpA phospholipase C which mediates rhabdomeric phototransduction. The Plcb4 -/- genotype, in addition to removing the intrinsic PLR, also essentially eliminates the intrinsic light response of the M1 subtype of melanopsin-expressing, intrinsically photosensitive retinal ganglion cells (M1-ipRGCs), which are by far the most photosensitive ipRGC subtype and also have the largest response to light. Ablating in mouse the expression of both TRPC6 and TRPC7, members of the TRP channel superfamily, also essentially eliminated the M1-ipRGC light response but the intrinsic PLR was not affected. Thus, melanopsin signalling exists in both iris and retina, involving a PLCβ4-mediated pathway that nonetheless diverges in the two locations.

Original languageEnglish (US)
Pages (from-to)67-72
Number of pages6
JournalNature
Volume479
Issue number7371
DOIs
StatePublished - Nov 3 2011

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Iris
Pupillary Reflex
Retina
Light
Vertebrates
Mammals
Light Signal Transduction
melanopsin
Retinal Pigments
Retinal Ganglion Cells
Type C Phospholipases
Drosophila
Genotype
Brain

ASJC Scopus subject areas

  • General

Cite this

Xue, T., Do, M. T. H., Riccio, A., Jiang, Z., Hsieh, J., Wang, H. C., ... Yau, K-W. (2011). Melanopsin signalling in mammalian iris and retina. Nature, 479(7371), 67-72. https://doi.org/10.1038/nature10567

Melanopsin signalling in mammalian iris and retina. / Xue, T.; Do, M. T H; Riccio, A.; Jiang, Zheng; Hsieh, J.; Wang, H. C.; Merbs, Shannath L; Welsbie, D. S.; Yoshioka, T.; Weissgerber, P.; Stolz, S.; Flockerzi, V.; Freichel, M.; Simon, M. I.; Clapham, D. E.; Yau, King-Wai.

In: Nature, Vol. 479, No. 7371, 03.11.2011, p. 67-72.

Research output: Contribution to journalArticle

Xue, T, Do, MTH, Riccio, A, Jiang, Z, Hsieh, J, Wang, HC, Merbs, SL, Welsbie, DS, Yoshioka, T, Weissgerber, P, Stolz, S, Flockerzi, V, Freichel, M, Simon, MI, Clapham, DE & Yau, K-W 2011, 'Melanopsin signalling in mammalian iris and retina', Nature, vol. 479, no. 7371, pp. 67-72. https://doi.org/10.1038/nature10567
Xue T, Do MTH, Riccio A, Jiang Z, Hsieh J, Wang HC et al. Melanopsin signalling in mammalian iris and retina. Nature. 2011 Nov 3;479(7371):67-72. https://doi.org/10.1038/nature10567
Xue, T. ; Do, M. T H ; Riccio, A. ; Jiang, Zheng ; Hsieh, J. ; Wang, H. C. ; Merbs, Shannath L ; Welsbie, D. S. ; Yoshioka, T. ; Weissgerber, P. ; Stolz, S. ; Flockerzi, V. ; Freichel, M. ; Simon, M. I. ; Clapham, D. E. ; Yau, King-Wai. / Melanopsin signalling in mammalian iris and retina. In: Nature. 2011 ; Vol. 479, No. 7371. pp. 67-72.
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