Melanoma mouse model implicates metabotropic glutamate signaling in melanocytic neoplasia

Pamela M. Pollock, Karine Cohen-Solal, Raman Sood, Jin Namkoong, Jeffrey J. Martino, Aruna Koganti, Hua Zhu, Christiane Robbins, Izabela Makalowska, Seung Shick Shin, Yari Marin, Kathleen G. Roberts, Laura M. Yudt, Amy Chen, Jun Cheng, Arturo Incao, Heather W. Pinkett, Christopher L. Graham, Karen Dunn, Steven M. Crespo-CarboneKerine R. Mackason, Kevin B. Ryan, Daniel Sinsimer, James Goydos, Kenneth R. Reuhl, Michael Eckhaus, Paul S. Meltzer, William J. Pavan, Jeffrey M. Trent, Suzie Chen

Research output: Contribution to journalArticle

Abstract

To gain insight into melanoma pathogenesis, we characterized an insertional mouse mutant, TG3, that is predisposed to develop multiple melanomas. Physical mapping identified multiple tandem insertions of the transgene into intron 3 of Grml (encoding metabotropic glutamate receptor 1) with concomitant deletion of 70 kb of intronic sequence. To assess whether this insertional mutagenesis event results in alteration of transcriptional regulation, we analyzed Grm1 and two flanking genes for aberrant expression in melanomas from TG3 mice. We observed aberrant expression of only Grm1. Although we did not detect its expression in normal mouse melanocytes, Grm1 was ectopically expressed in the melanomas from TG3 mice. To confirm the involvement of Grm1 in melanocytic neoplasia, we created an additional transgenic line with Grm1 expression driven by the dopachrome tautomerase promoter. Similar to the original TG3, the Tg(Grm1)EPv line was susceptible to melanoma. In contrast to human melanoma, these transgenic mice had a generalized hyperproliferation of melanocytes with limited transformation to fully malignant metastasis. We detected expression of GRM1 in a number of human melanoma biopsies and cell lines but not in benign nevi and melanocytes. This study provides compelling evidence for the importance of metabotropic glutamate signaling in melanocytic neoplasia.

Original languageEnglish (US)
Pages (from-to)108-112
Number of pages5
JournalNature Genetics
Volume34
Issue number1
DOIs
StatePublished - May 1 2003
Externally publishedYes

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Glutamic Acid
Melanoma
Melanocytes
Neoplasms
Insertional Mutagenesis
Nevus
Transgenes
Introns
Transgenic Mice
Neoplasm Metastasis
Biopsy
Gene Expression
Cell Line

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics

Cite this

Pollock, P. M., Cohen-Solal, K., Sood, R., Namkoong, J., Martino, J. J., Koganti, A., ... Chen, S. (2003). Melanoma mouse model implicates metabotropic glutamate signaling in melanocytic neoplasia. Nature Genetics, 34(1), 108-112. https://doi.org/10.1038/ng1148

Melanoma mouse model implicates metabotropic glutamate signaling in melanocytic neoplasia. / Pollock, Pamela M.; Cohen-Solal, Karine; Sood, Raman; Namkoong, Jin; Martino, Jeffrey J.; Koganti, Aruna; Zhu, Hua; Robbins, Christiane; Makalowska, Izabela; Shin, Seung Shick; Marin, Yari; Roberts, Kathleen G.; Yudt, Laura M.; Chen, Amy; Cheng, Jun; Incao, Arturo; Pinkett, Heather W.; Graham, Christopher L.; Dunn, Karen; Crespo-Carbone, Steven M.; Mackason, Kerine R.; Ryan, Kevin B.; Sinsimer, Daniel; Goydos, James; Reuhl, Kenneth R.; Eckhaus, Michael; Meltzer, Paul S.; Pavan, William J.; Trent, Jeffrey M.; Chen, Suzie.

In: Nature Genetics, Vol. 34, No. 1, 01.05.2003, p. 108-112.

Research output: Contribution to journalArticle

Pollock, PM, Cohen-Solal, K, Sood, R, Namkoong, J, Martino, JJ, Koganti, A, Zhu, H, Robbins, C, Makalowska, I, Shin, SS, Marin, Y, Roberts, KG, Yudt, LM, Chen, A, Cheng, J, Incao, A, Pinkett, HW, Graham, CL, Dunn, K, Crespo-Carbone, SM, Mackason, KR, Ryan, KB, Sinsimer, D, Goydos, J, Reuhl, KR, Eckhaus, M, Meltzer, PS, Pavan, WJ, Trent, JM & Chen, S 2003, 'Melanoma mouse model implicates metabotropic glutamate signaling in melanocytic neoplasia', Nature Genetics, vol. 34, no. 1, pp. 108-112. https://doi.org/10.1038/ng1148
Pollock PM, Cohen-Solal K, Sood R, Namkoong J, Martino JJ, Koganti A et al. Melanoma mouse model implicates metabotropic glutamate signaling in melanocytic neoplasia. Nature Genetics. 2003 May 1;34(1):108-112. https://doi.org/10.1038/ng1148
Pollock, Pamela M. ; Cohen-Solal, Karine ; Sood, Raman ; Namkoong, Jin ; Martino, Jeffrey J. ; Koganti, Aruna ; Zhu, Hua ; Robbins, Christiane ; Makalowska, Izabela ; Shin, Seung Shick ; Marin, Yari ; Roberts, Kathleen G. ; Yudt, Laura M. ; Chen, Amy ; Cheng, Jun ; Incao, Arturo ; Pinkett, Heather W. ; Graham, Christopher L. ; Dunn, Karen ; Crespo-Carbone, Steven M. ; Mackason, Kerine R. ; Ryan, Kevin B. ; Sinsimer, Daniel ; Goydos, James ; Reuhl, Kenneth R. ; Eckhaus, Michael ; Meltzer, Paul S. ; Pavan, William J. ; Trent, Jeffrey M. ; Chen, Suzie. / Melanoma mouse model implicates metabotropic glutamate signaling in melanocytic neoplasia. In: Nature Genetics. 2003 ; Vol. 34, No. 1. pp. 108-112.
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