Melanization affects susceptibility of Cryptococcus neoformans to heat and cold

Ángel Luis Rosas, Arturo Casadevall

Research output: Contribution to journalArticle

Abstract

Cryptococcus neoformans can synthesize melanin from a variety of substrates, including L-dopa (L-3,4-dihydroxyphenylalanine). Growth in minimal medium with L-dopa resulted in progressive accumulation of melanin in stationary phase cells. Melanized and non-melanized yeast cells were exposed to heat (42-47°C) and cold (-20°C), and the percentage of survival determined. Melanized cells were less susceptible to heat than non-melanized cells of the same age. Melanized cells from early stationary phase cultures were less susceptible to cold than non-melanized cells of the same age. However, melanized cells from late stationary phase cultures were more susceptible to cold than non-melanized cells of the same age. There was no statistical difference in susceptibility to heat and cold between melanin- deficient cells grown with and without L-dopa. These results suggest a role for melanin in protection against heat and cold.

Original languageEnglish (US)
Pages (from-to)265-272
Number of pages8
JournalFEMS Microbiology Letters
Volume153
Issue number2
DOIs
StatePublished - Aug 15 1997
Externally publishedYes

Fingerprint

Cryptococcus neoformans
Hot Temperature
Melanins
Levodopa
Yeasts

Keywords

  • Cryptococcus neoformans
  • Melanin
  • Phenoloxidase
  • Thermoprotection

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Melanization affects susceptibility of Cryptococcus neoformans to heat and cold. / Rosas, Ángel Luis; Casadevall, Arturo.

In: FEMS Microbiology Letters, Vol. 153, No. 2, 15.08.1997, p. 265-272.

Research output: Contribution to journalArticle

@article{cd3c8cd97b57491ab38768fc085bc3f6,
title = "Melanization affects susceptibility of Cryptococcus neoformans to heat and cold",
abstract = "Cryptococcus neoformans can synthesize melanin from a variety of substrates, including L-dopa (L-3,4-dihydroxyphenylalanine). Growth in minimal medium with L-dopa resulted in progressive accumulation of melanin in stationary phase cells. Melanized and non-melanized yeast cells were exposed to heat (42-47°C) and cold (-20°C), and the percentage of survival determined. Melanized cells were less susceptible to heat than non-melanized cells of the same age. Melanized cells from early stationary phase cultures were less susceptible to cold than non-melanized cells of the same age. However, melanized cells from late stationary phase cultures were more susceptible to cold than non-melanized cells of the same age. There was no statistical difference in susceptibility to heat and cold between melanin- deficient cells grown with and without L-dopa. These results suggest a role for melanin in protection against heat and cold.",
keywords = "Cryptococcus neoformans, Melanin, Phenoloxidase, Thermoprotection",
author = "Rosas, {{\'A}ngel Luis} and Arturo Casadevall",
year = "1997",
month = "8",
day = "15",
doi = "10.1016/S0378-1097(97)00239-5",
language = "English (US)",
volume = "153",
pages = "265--272",
journal = "FEMS Microbiology Letters",
issn = "0378-1097",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Melanization affects susceptibility of Cryptococcus neoformans to heat and cold

AU - Rosas, Ángel Luis

AU - Casadevall, Arturo

PY - 1997/8/15

Y1 - 1997/8/15

N2 - Cryptococcus neoformans can synthesize melanin from a variety of substrates, including L-dopa (L-3,4-dihydroxyphenylalanine). Growth in minimal medium with L-dopa resulted in progressive accumulation of melanin in stationary phase cells. Melanized and non-melanized yeast cells were exposed to heat (42-47°C) and cold (-20°C), and the percentage of survival determined. Melanized cells were less susceptible to heat than non-melanized cells of the same age. Melanized cells from early stationary phase cultures were less susceptible to cold than non-melanized cells of the same age. However, melanized cells from late stationary phase cultures were more susceptible to cold than non-melanized cells of the same age. There was no statistical difference in susceptibility to heat and cold between melanin- deficient cells grown with and without L-dopa. These results suggest a role for melanin in protection against heat and cold.

AB - Cryptococcus neoformans can synthesize melanin from a variety of substrates, including L-dopa (L-3,4-dihydroxyphenylalanine). Growth in minimal medium with L-dopa resulted in progressive accumulation of melanin in stationary phase cells. Melanized and non-melanized yeast cells were exposed to heat (42-47°C) and cold (-20°C), and the percentage of survival determined. Melanized cells were less susceptible to heat than non-melanized cells of the same age. Melanized cells from early stationary phase cultures were less susceptible to cold than non-melanized cells of the same age. However, melanized cells from late stationary phase cultures were more susceptible to cold than non-melanized cells of the same age. There was no statistical difference in susceptibility to heat and cold between melanin- deficient cells grown with and without L-dopa. These results suggest a role for melanin in protection against heat and cold.

KW - Cryptococcus neoformans

KW - Melanin

KW - Phenoloxidase

KW - Thermoprotection

UR - http://www.scopus.com/inward/record.url?scp=0030832492&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030832492&partnerID=8YFLogxK

U2 - 10.1016/S0378-1097(97)00239-5

DO - 10.1016/S0378-1097(97)00239-5

M3 - Article

C2 - 9271852

AN - SCOPUS:0030832492

VL - 153

SP - 265

EP - 272

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 2

ER -