Meiotic cell cycle requirement for a fly homologue of human deleted in Azoospermia

C. G. Eberhart, J. Z. Maines, S. A. Wasserman

Research output: Contribution to journalArticlepeer-review

Abstract

Infertility resulting from a severe defect in sperm production affects 2% of men worldwide. Of these men with azoospermia, the absence of sperm in semen, one in eight carry de novo deletions for a specific region of the Y chromosome. A candidate gene for the Y-chromosome azoospermia factor (AZF) has been identified and named Deleted in Azoospermia (DAZ). Here we describe the cloning and characterization of the Drosophila gene boule, which is a homologue of DAZ. The two genes encode closely related proteins that contain a predicted RNA-binding motif, and both loci are expressed exclusively in the testis. Loss of boule function results in azoospermia; meiotic divisions are blocked, although limited spermatid differentiation occurs. Histological examination of boule testes with cell-cycle markers indicates that the primary defect is at the meiotic G2/M transition. These results support the hypothesis that DAZ is the human AZF, and indicate that Boule and DAZ have an essential meiotic function in fly and human spermatogenesis.

Original languageEnglish (US)
Pages (from-to)783-785
Number of pages3
JournalNature
Volume381
Issue number6585
DOIs
StatePublished - Aug 2 1996
Externally publishedYes

ASJC Scopus subject areas

  • General

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