Mediation of Epstein-Barr virus EBNA2 transactivation by recombination signal-binding protein Jκ

Thomas Henkel, Paul D. Ling, S. Diane Hayward, Michael Gregory Peterson

Research output: Contribution to journalArticle

Abstract

The Epstein-Barr virus (EBV) transactivator protein, termed Epstein-Barr virus nuclear antigen 2 (EBNA2), plays a critical role in the regulation of latent viral transcription and in the immortalization of EBV-infected B cells. Unlike most transcription factors, EBNA2 does not bind directly to its cis-responsive DNA element but requires a cellular factor, termed C-promoter binding factor 1 (CBF1). Here, CBF1 was purified and was found to directly interact with EBNA2. CBF1 is identical to a protein thought to be involved in immunoglobulin gene rearrangement, RBPJκ. Contrary to previous reports, CBF1-RBPJκ did not bind to the recombination signal sequences but instead bound to sites in the EBV C-promoter and in the CD23 promoter.

Original languageEnglish (US)
Pages (from-to)92-95
Number of pages4
JournalScience
Volume265
Issue number5168
StatePublished - Jul 1 1994

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Immunoglobulin J Recombination Signal Sequence-Binding Protein
Epstein-Barr Virus Nuclear Antigens
Human Herpesvirus 4
Transcriptional Activation
Immunoglobulin Genes
Trans-Activators
Gene Rearrangement
Protein Sorting Signals
Genetic Recombination
Proteins
B-Lymphocytes
Transcription Factors
DNA

ASJC Scopus subject areas

  • General

Cite this

Henkel, T., Ling, P. D., Hayward, S. D., & Peterson, M. G. (1994). Mediation of Epstein-Barr virus EBNA2 transactivation by recombination signal-binding protein Jκ . Science, 265(5168), 92-95.

Mediation of Epstein-Barr virus EBNA2 transactivation by recombination signal-binding protein Jκ . / Henkel, Thomas; Ling, Paul D.; Hayward, S. Diane; Peterson, Michael Gregory.

In: Science, Vol. 265, No. 5168, 01.07.1994, p. 92-95.

Research output: Contribution to journalArticle

Henkel, T, Ling, PD, Hayward, SD & Peterson, MG 1994, 'Mediation of Epstein-Barr virus EBNA2 transactivation by recombination signal-binding protein Jκ ', Science, vol. 265, no. 5168, pp. 92-95.
Henkel T, Ling PD, Hayward SD, Peterson MG. Mediation of Epstein-Barr virus EBNA2 transactivation by recombination signal-binding protein Jκ . Science. 1994 Jul 1;265(5168):92-95.
Henkel, Thomas ; Ling, Paul D. ; Hayward, S. Diane ; Peterson, Michael Gregory. / Mediation of Epstein-Barr virus EBNA2 transactivation by recombination signal-binding protein Jκ . In: Science. 1994 ; Vol. 265, No. 5168. pp. 92-95.
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