Mechanosensitive channel stimulation system using low-intensity ultrasound by piezoelectric micromachined ultrasonic transducer array

Wonjun Lee; Samhwan Kim; Joontaek Jung; Woojin Kang; Eunjung Shin; Cheil Moon; Hongsoo Choi

doi:10.1109/ULTSYM.2016.7728651

Mechanosensitive channel stimulation system using low-intensity ultrasound by piezoelectric micromachined ultrasonic transducer array

Wonjun Lee, Samhwan Kim, Joontaek Jung, Woojin Kang, Eunjung Shin, Cheil Moon, Hongsoo Choi

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Scopus citations

Abstract

In this research, piezoelectric micromachined ultrasonic transducer (pMUT) array was used for stimulation of mechanosensitive channel of HEK293T cells. The fabricated pMUT array was made by microelectromechanical system (MEMS) technology such as photo process, etching, sputtering, and sol-gel method. The resonance frequency and acoustic positive peak pressure were 2.07 MHz and 3.94 kPa, respectively. The proposed system can be used for ultrasonic stimulation and the result can be confirmed using fluorescence microscope by observing the cation changing in real-time. The cells were treated by fluorescence staining and transient receptor potential cation channel subfamily V member 1 (TRPV1) channel inserting. The Ca⁺ ion level of stimulated cell was changed by ultrasound. The result showed mechanosensitive channel was activated by ultrasound stimulation and observed influx of Ca⁺ ion in real-time.

Original language	English (US)
Title of host publication	2016 IEEE International Ultrasonics Symposium, IUS 2016
Publisher	IEEE Computer Society
Volume	2016-November
ISBN (Electronic)	9781467398978
DOIs	https://doi.org/10.1109/ULTSYM.2016.7728651
State	Published - Nov 1 2016
Externally published	Yes
Event	2016 IEEE International Ultrasonics Symposium, IUS 2016 - Tours, France Duration: Sep 18 2016 → Sep 21 2016

Other

Other	2016 IEEE International Ultrasonics Symposium, IUS 2016
Country/Territory	France
City	Tours
Period	9/18/16 → 9/21/16

Keywords

Mechanosensitive channel
piezoelectric MEMS
pMUT
Ultrasonic stimulation

ASJC Scopus subject areas

Acoustics and Ultrasonics

Access to Document

10.1109/ULTSYM.2016.7728651

Cite this

Lee, W., Kim, S., Jung, J., Kang, W., Shin, E., Moon, C., & Choi, H. (2016). Mechanosensitive channel stimulation system using low-intensity ultrasound by piezoelectric micromachined ultrasonic transducer array. In 2016 IEEE International Ultrasonics Symposium, IUS 2016 (Vol. 2016-November). Article 7728651 IEEE Computer Society. https://doi.org/10.1109/ULTSYM.2016.7728651

Mechanosensitive channel stimulation system using low-intensity ultrasound by piezoelectric micromachined ultrasonic transducer array. / Lee, Wonjun; Kim, Samhwan; Jung, Joontaek et al.
2016 IEEE International Ultrasonics Symposium, IUS 2016. Vol. 2016-November IEEE Computer Society, 2016. 7728651.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Lee, W, Kim, S, Jung, J, Kang, W, Shin, E, Moon, C & Choi, H 2016, Mechanosensitive channel stimulation system using low-intensity ultrasound by piezoelectric micromachined ultrasonic transducer array. in 2016 IEEE International Ultrasonics Symposium, IUS 2016. vol. 2016-November, 7728651, IEEE Computer Society, 2016 IEEE International Ultrasonics Symposium, IUS 2016, Tours, France, 9/18/16. https://doi.org/10.1109/ULTSYM.2016.7728651

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abstract = "In this research, piezoelectric micromachined ultrasonic transducer (pMUT) array was used for stimulation of mechanosensitive channel of HEK293T cells. The fabricated pMUT array was made by microelectromechanical system (MEMS) technology such as photo process, etching, sputtering, and sol-gel method. The resonance frequency and acoustic positive peak pressure were 2.07 MHz and 3.94 kPa, respectively. The proposed system can be used for ultrasonic stimulation and the result can be confirmed using fluorescence microscope by observing the cation changing in real-time. The cells were treated by fluorescence staining and transient receptor potential cation channel subfamily V member 1 (TRPV1) channel inserting. The Ca+ ion level of stimulated cell was changed by ultrasound. The result showed mechanosensitive channel was activated by ultrasound stimulation and observed influx of Ca+ ion in real-time.",

keywords = "Mechanosensitive channel, piezoelectric MEMS, pMUT, Ultrasonic stimulation",

author = "Wonjun Lee and Samhwan Kim and Joontaek Jung and Woojin Kang and Eunjung Shin and Cheil Moon and Hongsoo Choi",

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AU - Moon, Cheil

AU - Choi, Hongsoo

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N2 - In this research, piezoelectric micromachined ultrasonic transducer (pMUT) array was used for stimulation of mechanosensitive channel of HEK293T cells. The fabricated pMUT array was made by microelectromechanical system (MEMS) technology such as photo process, etching, sputtering, and sol-gel method. The resonance frequency and acoustic positive peak pressure were 2.07 MHz and 3.94 kPa, respectively. The proposed system can be used for ultrasonic stimulation and the result can be confirmed using fluorescence microscope by observing the cation changing in real-time. The cells were treated by fluorescence staining and transient receptor potential cation channel subfamily V member 1 (TRPV1) channel inserting. The Ca+ ion level of stimulated cell was changed by ultrasound. The result showed mechanosensitive channel was activated by ultrasound stimulation and observed influx of Ca+ ion in real-time.

AB - In this research, piezoelectric micromachined ultrasonic transducer (pMUT) array was used for stimulation of mechanosensitive channel of HEK293T cells. The fabricated pMUT array was made by microelectromechanical system (MEMS) technology such as photo process, etching, sputtering, and sol-gel method. The resonance frequency and acoustic positive peak pressure were 2.07 MHz and 3.94 kPa, respectively. The proposed system can be used for ultrasonic stimulation and the result can be confirmed using fluorescence microscope by observing the cation changing in real-time. The cells were treated by fluorescence staining and transient receptor potential cation channel subfamily V member 1 (TRPV1) channel inserting. The Ca+ ion level of stimulated cell was changed by ultrasound. The result showed mechanosensitive channel was activated by ultrasound stimulation and observed influx of Ca+ ion in real-time.

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KW - Ultrasonic stimulation

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Mechanosensitive channel stimulation system using low-intensity ultrasound by piezoelectric micromachined ultrasonic transducer array

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