Mechanisms subserving insulin action in the gonad: Evidence that insulin induces specific phosphorylation of its immunoprecipitable receptor on ovarian cells

Tohannes D. Veldhuis, Shinri Tamura, Lisa Kolp, Richard W. Furlanetto, Joseph Larner

Research output: Contribution to journalArticlepeer-review

Abstract

To test the capacity of insulin to work through a classical insulin-receptor pathway in the ovary, cultured swine granulosa cells were treated with insulin and/or increasing concentrations of insulin-receptor antiserum. Insulin-receptor antiserum but not control serum significantly (>85%) attenuated insulin's stimulation of progesterone biosynthesis. Moreover, in broken-cell preparations, insulin but not desoctapeptide insulin or somatomedins induced specific phosphorylation of the 95,000-dalton, immunoprecipitated beta subunit of the insulin receptor on ovarian cells. These observations provide the first evidence for discrete biochemical actions of insulin at the level of the cell-membrane receptor for insulin in gonadal cells.

Original languageEnglish (US)
Pages (from-to)144-149
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume120
Issue number1
DOIs
StatePublished - Apr 16 1984

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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