Mechanisms of prostanoid synthesis in human synovial cells

Cytokinepeptide synergism

Joan M. Bathon, Floyd H. Chilton, Walter C. Hubbard, Marilyn C. Towns, Nancie J. Solan, David Proud

Research output: Contribution to journalArticle

Abstract

Bradykinin (BK)2 and interleukin-1 (IL-1) interact synergistically to stimulate prostaglandin synthesis in human synovial fibroblast-like cells. The effect of BK is rapid and correlates with its capacity to elevate cytosolic levels of calcium ([Ca2+](i)), while IL-1's effect is slow and is dependent upon de novo protein synthesis. The mechanism of this synergistic interaction was investigated. In the basal state, high levels of arachidonic acid (AA) were spontaneously released from synovial cells but near absent levels of cyclooxygenase activity prevented metabolism of AA to prostanoid. BK was a potent stimulus for elevating AA, but not prostaglandins, above basal levels. IL-1, in contrast, increased prostaglandins, but not AA, above basal levels. IL-1 treatment was not associated with a loss or redistribution of AA among phospholipid classes. These results are consistent with high basal phospholipase activity in synovial cells and demonstrate the ability of BK, presumably via its ability to raise [Ca2+](i), to further elevate this activity(ies). Metabolism of AA to prostanoid is minimal in resting and BK-stimulated synovial cells, however, without the concomitant induction of cyclooxygenase activity by IL-1. These studies clarify the different, but synergistic, mechanisms of action of a peptide and cytokine in stimulating prostanoid synthesis in synovial cells. In addition, these data extend the results of previous investigations in demonstrating that basal phospholipase activity provides sufficient AA substrate for IL-1 induced prostanoid synthesis without invoking the concomitant induction of phospholipase activity by IL-1.

Original languageEnglish (US)
Pages (from-to)537-554
Number of pages18
JournalInflammation
Volume20
Issue number5
DOIs
StatePublished - 1996

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Interleukin-1
Arachidonic Acid
Prostaglandins
Bradykinin
Phospholipases
Prostaglandin-Endoperoxide Synthases
Phospholipids
Fibroblasts
Cytokines
Calcium
Peptides
Proteins

ASJC Scopus subject areas

  • Medicine(all)
  • Immunology
  • Cell Biology

Cite this

Bathon, J. M., Chilton, F. H., Hubbard, W. C., Towns, M. C., Solan, N. J., & Proud, D. (1996). Mechanisms of prostanoid synthesis in human synovial cells: Cytokinepeptide synergism. Inflammation, 20(5), 537-554. https://doi.org/10.1007/BF01487045

Mechanisms of prostanoid synthesis in human synovial cells : Cytokinepeptide synergism. / Bathon, Joan M.; Chilton, Floyd H.; Hubbard, Walter C.; Towns, Marilyn C.; Solan, Nancie J.; Proud, David.

In: Inflammation, Vol. 20, No. 5, 1996, p. 537-554.

Research output: Contribution to journalArticle

Bathon, JM, Chilton, FH, Hubbard, WC, Towns, MC, Solan, NJ & Proud, D 1996, 'Mechanisms of prostanoid synthesis in human synovial cells: Cytokinepeptide synergism', Inflammation, vol. 20, no. 5, pp. 537-554. https://doi.org/10.1007/BF01487045
Bathon JM, Chilton FH, Hubbard WC, Towns MC, Solan NJ, Proud D. Mechanisms of prostanoid synthesis in human synovial cells: Cytokinepeptide synergism. Inflammation. 1996;20(5):537-554. https://doi.org/10.1007/BF01487045
Bathon, Joan M. ; Chilton, Floyd H. ; Hubbard, Walter C. ; Towns, Marilyn C. ; Solan, Nancie J. ; Proud, David. / Mechanisms of prostanoid synthesis in human synovial cells : Cytokinepeptide synergism. In: Inflammation. 1996 ; Vol. 20, No. 5. pp. 537-554.
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