Mechanisms of amphotericin B-induced reduction of the glomerular filtration rate: A micropuncture study

R. Sabra, K. Takahashi, R. A. Branch, K. F. Badr

Research output: Contribution to journalArticlepeer-review

Abstract

Amphotericin B infusions cause acute reductions in renal plasma flow and glomerular filtration rate. The exact mechanism by which these changes occur has not been identified fully, nor have the effects of the drug on the renal microcirculation been studied adequately. In this study, we examined the effect of intrarenal amphotericin B infusions (0.05 mg/kg/min) on glomerular hemodynamics in the anesthetized rat. Amphotericin B did not affect systemic blood pressure and slightly increased hematocrit (5%), but significantly decreased renal blood flow and glomerular filtration rate by 40 and 35%, respectively. Glomerular micropuncture revealed decreases in single nephron plasma flow and glomerular filtration rate (from 142 ± 12 to 89 ± 14 and from 35.3 ± 2.2 to 22.8 ± 2.8 nl/min, respectively). These changes were due to significant increases in pre- and postglomerular resistances (from 1.91 ± 0.17 to 3.95 ± 0.38 and from 1.30 ± 0.10 to 2.08 ± 0.12 1010dyn · sec · cm-5, respectively), and to a significant decrease in the glomerular capillary ultrafiltration coeffcient which fell from 0.043 ± 0.008 to 0.032 ± 0.009 nl/(sec · mm Hg). These results provide further insight into the mechanisms of the acute renal effects of amphotericin B, and suggest possible mediators that may be involved in these effects.

Original languageEnglish (US)
Pages (from-to)34-37
Number of pages4
JournalJournal of Pharmacology and Experimental Therapeutics
Volume253
Issue number1
StatePublished - 1990
Externally publishedYes

ASJC Scopus subject areas

  • Pharmacology

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