Mechanism of chromatin remodeling and recovery during passage of RNA polymerase II

Olga I. Kulaeva, Daria A. Gaykalova, Nikolai A. Pestov, Viktor V. Golovastov, Dmitry G. Vassylyev, Irina Artsimovitch, Vasily M. Studitsky

Research output: Contribution to journalArticlepeer-review

Abstract

Transcription of eukaryotic genes by RNA polymerase II (Pol II) is typically accompanied by nucleosome survival and minimal exchange of histones H3 and H4. The mechanism of nucleosome survival and recovery of chromatin structure remains obscure. Here we show how transcription through chromatin by Pol II is uniquely coupled with nucleosome survival. Structural modeling and functional analysis of the intermediates of transcription through a nucleosome indicated that when Pol II approaches an area of strong DNA-histone interactions, a small intranucleosomal DNA loop (zero-size or-loop) containing transcribing enzyme is formed. During formation of the-loop, the recovery of DNA-histone interactions behind Pol II is tightly coupled with their disruption ahead of the enzyme. This coupling is a distinct feature of the Pol II-type mechanism that allows further transcription through the nucleosome, prevents nucleosome translocation and minimizes displacement of H3 and H4 histones from DNA during enzyme passage.

Original languageEnglish (US)
Pages (from-to)1272-1278
Number of pages7
JournalNature Structural and Molecular Biology
Volume16
Issue number12
DOIs
StatePublished - Dec 2009

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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