Mechanism of action of mineral oil adjuvants. I. Conditions influencing the in vitro stability of water-in-oil emulsions

Paul Steinberg, Philip S. Norman

Research output: Contribution to journalArticle

Abstract

An experimental model has been developed in which the stability of water-in-oil emulsions in simple environments of known constitution could be studied. A decrease in emulsion stability was effected by decreasing the pH of the emulsion environment with the ionic strength constant, increasing the ionic strength of the environment with the pH constant, or increasing the temperature of the environment with the pH and ionic strength constant. The concentration of protein in the emulsion environment at a given pH was not found to alter the stability of the emulsions. Parallels between in vitro influences upon stability and those produced by an inflammatory response at an emulsion repository site are discussed.

Original languageEnglish (US)
Pages (from-to)238-244
Number of pages7
JournalThe Journal of Allergy and Clinical Immunology
Volume51
Issue number4
DOIs
StatePublished - 1973
Externally publishedYes

Fingerprint

Mineral Oil
Emulsions
Oils
Water
Osmolar Concentration
Constitution and Bylaws
In Vitro Techniques
Theoretical Models
Temperature
Proteins

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

@article{3fe0e97afe40457fafe2f35f128121e2,
title = "Mechanism of action of mineral oil adjuvants. I. Conditions influencing the in vitro stability of water-in-oil emulsions",
abstract = "An experimental model has been developed in which the stability of water-in-oil emulsions in simple environments of known constitution could be studied. A decrease in emulsion stability was effected by decreasing the pH of the emulsion environment with the ionic strength constant, increasing the ionic strength of the environment with the pH constant, or increasing the temperature of the environment with the pH and ionic strength constant. The concentration of protein in the emulsion environment at a given pH was not found to alter the stability of the emulsions. Parallels between in vitro influences upon stability and those produced by an inflammatory response at an emulsion repository site are discussed.",
author = "Paul Steinberg and Norman, {Philip S.}",
year = "1973",
doi = "10.1016/0091-6749(73)90143-7",
language = "English (US)",
volume = "51",
pages = "238--244",
journal = "Journal of Allergy and Clinical Immunology",
issn = "0091-6749",
publisher = "Mosby Inc.",
number = "4",

}

TY - JOUR

T1 - Mechanism of action of mineral oil adjuvants. I. Conditions influencing the in vitro stability of water-in-oil emulsions

AU - Steinberg, Paul

AU - Norman, Philip S.

PY - 1973

Y1 - 1973

N2 - An experimental model has been developed in which the stability of water-in-oil emulsions in simple environments of known constitution could be studied. A decrease in emulsion stability was effected by decreasing the pH of the emulsion environment with the ionic strength constant, increasing the ionic strength of the environment with the pH constant, or increasing the temperature of the environment with the pH and ionic strength constant. The concentration of protein in the emulsion environment at a given pH was not found to alter the stability of the emulsions. Parallels between in vitro influences upon stability and those produced by an inflammatory response at an emulsion repository site are discussed.

AB - An experimental model has been developed in which the stability of water-in-oil emulsions in simple environments of known constitution could be studied. A decrease in emulsion stability was effected by decreasing the pH of the emulsion environment with the ionic strength constant, increasing the ionic strength of the environment with the pH constant, or increasing the temperature of the environment with the pH and ionic strength constant. The concentration of protein in the emulsion environment at a given pH was not found to alter the stability of the emulsions. Parallels between in vitro influences upon stability and those produced by an inflammatory response at an emulsion repository site are discussed.

UR - http://www.scopus.com/inward/record.url?scp=0015610069&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0015610069&partnerID=8YFLogxK

U2 - 10.1016/0091-6749(73)90143-7

DO - 10.1016/0091-6749(73)90143-7

M3 - Article

C2 - 4689140

AN - SCOPUS:0015610069

VL - 51

SP - 238

EP - 244

JO - Journal of Allergy and Clinical Immunology

JF - Journal of Allergy and Clinical Immunology

SN - 0091-6749

IS - 4

ER -