Mechanism of action of Micrococcus luteus γ-endonuclease

Timothy J. Jorgensen, Yoke Wah Kow, Susan S. Wallace, William D. Henner

Research output: Contribution to journalArticle

Abstract

Micrococcus luteus extracts contain γ-endonuclease, a Mg2+-independent endonuclease that cleaves γ-irradiated DNA. This enzyme has been purified approximately 1000-fold, and the purified enzyme was used to study its substrate specificity and mechanism of action. γ-Endonuclease cleaves DNA containing either thymine glycols, urea residues, or apurinic sites but not undamaged DNA or DNA containing reduced apurinic sites. The enzyme has both N-glycosylase activity that releases thymine glycol residues from OsO4-treated DNA and an associated apurinic endonuclease activity. The location and nature of the cleavage site produced has been determined with DNA sequencing techniques. γ-Endonuclease cleaves DNA containing thymine glycols or apurinic sites immediately 3′ to the damaged or missing base. Cleavage results in a 5′-phosphate terminus and a 3′ baseless sugar residue. Cleavage sites can be converted to primers for DNA polymerase I by subsequent treatment with Escherichia coli exonuclease III. The mechanism of action of γ-endonuclease and its substrate specificity are very similar to those identified for E. coli endonuclease III.

Original languageEnglish (US)
Pages (from-to)6436-6443
Number of pages8
JournalBiochemistry®
Volume26
Issue number20
StatePublished - 1987
Externally publishedYes

Fingerprint

Micrococcus luteus
Endonucleases
Deoxyribonuclease I
Substrate Specificity
DNA
Enzymes
DNA-(Apurinic or Apyrimidinic Site) Lyase
DNA Polymerase I
DNA Sequence Analysis
Urea
Phosphates
Escherichia coli
Substrates
Sugars
thymine glycol

ASJC Scopus subject areas

  • Biochemistry

Cite this

Jorgensen, T. J., Kow, Y. W., Wallace, S. S., & Henner, W. D. (1987). Mechanism of action of Micrococcus luteus γ-endonuclease. Biochemistry®, 26(20), 6436-6443.

Mechanism of action of Micrococcus luteus γ-endonuclease. / Jorgensen, Timothy J.; Kow, Yoke Wah; Wallace, Susan S.; Henner, William D.

In: Biochemistry®, Vol. 26, No. 20, 1987, p. 6436-6443.

Research output: Contribution to journalArticle

Jorgensen, TJ, Kow, YW, Wallace, SS & Henner, WD 1987, 'Mechanism of action of Micrococcus luteus γ-endonuclease', Biochemistry®, vol. 26, no. 20, pp. 6436-6443.
Jorgensen TJ, Kow YW, Wallace SS, Henner WD. Mechanism of action of Micrococcus luteus γ-endonuclease. Biochemistry®. 1987;26(20):6436-6443.
Jorgensen, Timothy J. ; Kow, Yoke Wah ; Wallace, Susan S. ; Henner, William D. / Mechanism of action of Micrococcus luteus γ-endonuclease. In: Biochemistry®. 1987 ; Vol. 26, No. 20. pp. 6436-6443.
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