Mechanism for the Regulated Control of Bacterial Transcription Termination by a Universal Adaptor Protein

Michael R. Lawson, Wen Ma, Michael J. Bellecourt, Irina Artsimovitch, Andreas Martin, Robert Landick, Klaus Schulten, James M. Berger

Research output: Contribution to journalArticlepeer-review

Abstract

NusG/Spt5 proteins are the only transcription factors utilized by all cellular organisms. In enterobacteria, NusG antagonizes the transcription termination activity of Rho, a hexameric helicase, during the synthesis of ribosomal and actively translated mRNAs. Paradoxically, NusG helps Rho act on untranslated transcripts, including non-canonical antisense RNAs and those arising from translational stress; how NusG fulfills these disparate functions is unknown. Here, we demonstrate that NusG activates Rho by assisting helicase isomerization from an open-ring, RNA-loading state to a closed-ring, catalytically active translocase. A crystal structure of closed-ring Rho in complex with NusG reveals the physical basis for this activation and further explains how Rho is excluded from translationally competent RNAs. This study demonstrates how a universally conserved transcription factor acts to modulate the activity of a ring-shaped ATPase motor and establishes how the innate sequence bias of a termination factor can be modulated to silence pervasive, aberrant transcription. Lawson et al. show that NusG, a member of a universally conserved transcription factor family, helps isomerize the Rho transcription termination factor from an open-ring loading state to an active, closed-ring conformation. This action overrides the innate sequence bias of Rho to terminate aberrant transcriptional events emanating from translational stress.

Original languageEnglish (US)
Pages (from-to)911-922.e4
JournalMolecular cell
Volume71
Issue number6
DOIs
StatePublished - Sep 20 2018

Keywords

  • ATPase
  • NusG
  • Rho
  • Spt5
  • termination
  • transcription
  • translation

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Mechanism for the Regulated Control of Bacterial Transcription Termination by a Universal Adaptor Protein'. Together they form a unique fingerprint.

Cite this