Mechanics of post-fusion exocytotic vesicle

Thomas Stephens, Zhanghan Wu, Jian Liu

Research output: Contribution to journalArticle

Abstract

Exocytosis is an important cellular process controlled by metabolic signaling. It involves vesicle fusion to the plasma membrane, followed by the opening of a fusion pore, and the subsequent release of the vesicular lumen content into the extracellular space. While most modeling efforts focus on the events leading to membrane fusion, how the vesicular membrane remodels after fusing to plasma membrane remains unclear. This latter event dictates the nature and the efficiency of exocytotic vesicular secretions, and is thus critical for exocytotic function. We provide a generic membrane mechanical model to systematically study the fate of post-fusion vesicles. We show that while membrane stiffness favors full-collapse vesicle fusion into the plasma membrane, the intravesicular pressure swells the vesicle and causes the fusion pore to shrink. Dimensions of the vesicle and its associated fusion pore further modulate this mechanical antagonism. We systematically define the mechanical conditions that account for the full spectrum of the observed vesicular secretion modes. Our model therefore can serve as a unified theoretical framework that sheds light on the elaborate control mechanism of exocytosis.

Original languageEnglish (US)
Article number035004
JournalPhysical Biology
Volume14
Issue number3
DOIs
StatePublished - May 23 2017
Externally publishedYes

Fingerprint

Mechanics
Exocytosis
Cell Membrane
Membranes
Membrane Fusion
Extracellular Space
Pressure

Keywords

  • exocytotic vesicle
  • membrane mechanical model
  • post-fusion
  • vesicle fusion

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Molecular Biology
  • Cell Biology

Cite this

Mechanics of post-fusion exocytotic vesicle. / Stephens, Thomas; Wu, Zhanghan; Liu, Jian.

In: Physical Biology, Vol. 14, No. 3, 035004, 23.05.2017.

Research output: Contribution to journalArticle

Stephens, Thomas ; Wu, Zhanghan ; Liu, Jian. / Mechanics of post-fusion exocytotic vesicle. In: Physical Biology. 2017 ; Vol. 14, No. 3.
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