Measuring the Physiologic Properties of Oral Lesions Receiving Fractionated Photodynamic Therapy

Shannon M. Gallagher-Colombo, Harry Quon, Kelly M. Malloy, Peter H. Ahn, Keith A. Cengel, Charles B. Simone, Ara A. Chalian, Bert W. O'Malley, Gregory S. Weinstein, Timothy C. Zhu, Mary E. Putt, Jarod C. Finlay, Theresa M. Busch

Research output: Contribution to journalArticle

Abstract

Photodynamic therapy (PDT) can treat superficial, early-stage disease with minimal damage to underlying tissues and without cumulative dose-limiting toxicity. Treatment efficacy is affected by disease physiologic properties, but these properties are not routinely measured. We assessed diffuse reflectance spectroscopy (DRS) for the noninvasive, contact measurement of tissue hemoglobin oxygen saturation (StO2) and total hemoglobin concentration ([tHb]) in the premalignant or superficial microinvasive oral lesions of patients treated with 5-aminolevulinic acid (ALA)-PDT. Patients were enrolled on a Phase 1 study of ALA-PDT that evaluated fluences of 50, 100, 150 or 200 J cm-2 delivered at 100 mW cm-2. To test the feasibility of incorporating DRS measurements within the illumination period, studies were performed in patients who received fractionated (two-part) illumination that included a dark interval of 90-180 s. Using DRS, tissue oxygenation at different depths within the lesion could also be assessed. DRS could be performed concurrently with contact measurements of photosensitizer levels by fluorescence spectroscopy, but a separate noncontact fluorescence spectroscopy system provided continuous assessment of photobleaching during illumination to greater tissue depths. Results establish that the integration of DRS into PDT of early-stage oral disease is feasible, and motivates further studies to evaluate its predictive and dosimetric value.

Original languageEnglish (US)
Pages (from-to)1210-1218
Number of pages9
JournalPhotochemistry and Photobiology
Volume91
Issue number5
DOIs
StatePublished - Sep 1 2015
Externally publishedYes

Fingerprint

Photodynamic therapy
Photochemotherapy
lesions
therapy
Spectrum Analysis
Spectroscopy
Lighting
Tissue
Aminolevulinic Acid
reflectance
spectroscopy
Fluorescence Spectrometry
Fluorescence spectroscopy
Hemoglobin oxygen saturation
Hemoglobins
Mouth Diseases
illumination
hemoglobin
Photobleaching
Photosensitizing Agents

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Biochemistry
  • Medicine(all)

Cite this

Gallagher-Colombo, S. M., Quon, H., Malloy, K. M., Ahn, P. H., Cengel, K. A., Simone, C. B., ... Busch, T. M. (2015). Measuring the Physiologic Properties of Oral Lesions Receiving Fractionated Photodynamic Therapy. Photochemistry and Photobiology, 91(5), 1210-1218. https://doi.org/10.1111/php.12475

Measuring the Physiologic Properties of Oral Lesions Receiving Fractionated Photodynamic Therapy. / Gallagher-Colombo, Shannon M.; Quon, Harry; Malloy, Kelly M.; Ahn, Peter H.; Cengel, Keith A.; Simone, Charles B.; Chalian, Ara A.; O'Malley, Bert W.; Weinstein, Gregory S.; Zhu, Timothy C.; Putt, Mary E.; Finlay, Jarod C.; Busch, Theresa M.

In: Photochemistry and Photobiology, Vol. 91, No. 5, 01.09.2015, p. 1210-1218.

Research output: Contribution to journalArticle

Gallagher-Colombo, SM, Quon, H, Malloy, KM, Ahn, PH, Cengel, KA, Simone, CB, Chalian, AA, O'Malley, BW, Weinstein, GS, Zhu, TC, Putt, ME, Finlay, JC & Busch, TM 2015, 'Measuring the Physiologic Properties of Oral Lesions Receiving Fractionated Photodynamic Therapy', Photochemistry and Photobiology, vol. 91, no. 5, pp. 1210-1218. https://doi.org/10.1111/php.12475
Gallagher-Colombo, Shannon M. ; Quon, Harry ; Malloy, Kelly M. ; Ahn, Peter H. ; Cengel, Keith A. ; Simone, Charles B. ; Chalian, Ara A. ; O'Malley, Bert W. ; Weinstein, Gregory S. ; Zhu, Timothy C. ; Putt, Mary E. ; Finlay, Jarod C. ; Busch, Theresa M. / Measuring the Physiologic Properties of Oral Lesions Receiving Fractionated Photodynamic Therapy. In: Photochemistry and Photobiology. 2015 ; Vol. 91, No. 5. pp. 1210-1218.
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