Measuring the activity of leucine-rich repeat kinase 2: A kinase involved in parkinson's disease

Byoung Dae Lee, Xiaojie Li, Ted M Dawson, Valina Dawson

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Mutations in the LRRK2 (Leucine-Rich Repeat Kinase 2) gene are the most common cause of autosomal dominant Parkinson's disease. LRRK2 has multiple functional domains including a kinase domain. The kinase activity of LRRK2 is implicated in the pathogenesis of Parkinson's disease. Developing an assay to understand the mechanisms of LRRK2 kinase activity is important for the development of pharmacologic and therapeutic applications. Here, we describe how to measure in vitro LRRK2 kinase activity and its inhibition.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages45-54
Number of pages10
Volume795
DOIs
StatePublished - 2012

Publication series

NameMethods in Molecular Biology
Volume795
ISSN (Print)10643745

Fingerprint

Leucine
Parkinson Disease
Phosphotransferases
Mutation
Genes

Keywords

  • In vitro kinase assay
  • LRRK2
  • Parkinson's disease

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Lee, B. D., Li, X., Dawson, T. M., & Dawson, V. (2012). Measuring the activity of leucine-rich repeat kinase 2: A kinase involved in parkinson's disease. In Methods in Molecular Biology (Vol. 795, pp. 45-54). (Methods in Molecular Biology; Vol. 795). https://doi.org/10.1007/978-1-61779-337-0_3

Measuring the activity of leucine-rich repeat kinase 2 : A kinase involved in parkinson's disease. / Lee, Byoung Dae; Li, Xiaojie; Dawson, Ted M; Dawson, Valina.

Methods in Molecular Biology. Vol. 795 2012. p. 45-54 (Methods in Molecular Biology; Vol. 795).

Research output: Chapter in Book/Report/Conference proceedingChapter

Lee, BD, Li, X, Dawson, TM & Dawson, V 2012, Measuring the activity of leucine-rich repeat kinase 2: A kinase involved in parkinson's disease. in Methods in Molecular Biology. vol. 795, Methods in Molecular Biology, vol. 795, pp. 45-54. https://doi.org/10.1007/978-1-61779-337-0_3
Lee BD, Li X, Dawson TM, Dawson V. Measuring the activity of leucine-rich repeat kinase 2: A kinase involved in parkinson's disease. In Methods in Molecular Biology. Vol. 795. 2012. p. 45-54. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-61779-337-0_3
Lee, Byoung Dae ; Li, Xiaojie ; Dawson, Ted M ; Dawson, Valina. / Measuring the activity of leucine-rich repeat kinase 2 : A kinase involved in parkinson's disease. Methods in Molecular Biology. Vol. 795 2012. pp. 45-54 (Methods in Molecular Biology).
@inbook{a46a43a0077143fe92d5ad7a199fd1f1,
title = "Measuring the activity of leucine-rich repeat kinase 2: A kinase involved in parkinson's disease",
abstract = "Mutations in the LRRK2 (Leucine-Rich Repeat Kinase 2) gene are the most common cause of autosomal dominant Parkinson's disease. LRRK2 has multiple functional domains including a kinase domain. The kinase activity of LRRK2 is implicated in the pathogenesis of Parkinson's disease. Developing an assay to understand the mechanisms of LRRK2 kinase activity is important for the development of pharmacologic and therapeutic applications. Here, we describe how to measure in vitro LRRK2 kinase activity and its inhibition.",
keywords = "In vitro kinase assay, LRRK2, Parkinson's disease",
author = "Lee, {Byoung Dae} and Xiaojie Li and Dawson, {Ted M} and Valina Dawson",
year = "2012",
doi = "10.1007/978-1-61779-337-0_3",
language = "English (US)",
isbn = "9781617793363",
volume = "795",
series = "Methods in Molecular Biology",
pages = "45--54",
booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Measuring the activity of leucine-rich repeat kinase 2

T2 - A kinase involved in parkinson's disease

AU - Lee, Byoung Dae

AU - Li, Xiaojie

AU - Dawson, Ted M

AU - Dawson, Valina

PY - 2012

Y1 - 2012

N2 - Mutations in the LRRK2 (Leucine-Rich Repeat Kinase 2) gene are the most common cause of autosomal dominant Parkinson's disease. LRRK2 has multiple functional domains including a kinase domain. The kinase activity of LRRK2 is implicated in the pathogenesis of Parkinson's disease. Developing an assay to understand the mechanisms of LRRK2 kinase activity is important for the development of pharmacologic and therapeutic applications. Here, we describe how to measure in vitro LRRK2 kinase activity and its inhibition.

AB - Mutations in the LRRK2 (Leucine-Rich Repeat Kinase 2) gene are the most common cause of autosomal dominant Parkinson's disease. LRRK2 has multiple functional domains including a kinase domain. The kinase activity of LRRK2 is implicated in the pathogenesis of Parkinson's disease. Developing an assay to understand the mechanisms of LRRK2 kinase activity is important for the development of pharmacologic and therapeutic applications. Here, we describe how to measure in vitro LRRK2 kinase activity and its inhibition.

KW - In vitro kinase assay

KW - LRRK2

KW - Parkinson's disease

UR - http://www.scopus.com/inward/record.url?scp=80555144278&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80555144278&partnerID=8YFLogxK

U2 - 10.1007/978-1-61779-337-0_3

DO - 10.1007/978-1-61779-337-0_3

M3 - Chapter

C2 - 21960214

AN - SCOPUS:80555144278

SN - 9781617793363

VL - 795

T3 - Methods in Molecular Biology

SP - 45

EP - 54

BT - Methods in Molecular Biology

ER -