Measuring JHH values with a selective constant-time 2D NMR protocol

Liangjie Lin, Zhiliang Wei, Yanqin Lin, Zhong Chen

Research output: Contribution to journalArticle

Abstract

Proton-proton scalar couplings play important roles in molecule structure elucidation. However, measurements of JHH values in complex coupled spin systems remain challenging. In this study, we develop a selective constant-time (SECT) 2D NMR protocol with which scalar coupling networks involving chosen protons can be revealed, and corresponding JHH values can be measured through doublets along the F1 dimension. All JHH values within a network of n fully coupled protons can be separately determined with (n − 1) SECT experiments. Additionally, the proposed pulse sequence possesses satisfactory sensitivity and handy implementation. Therefore, it will interest scientists who intend to address structural analyzes of molecules with overcrowded spectra, and may greatly facilitate the applications of scalar-coupling constants in molecule structure studies.

Original languageEnglish (US)
Pages (from-to)20-24
Number of pages5
JournalJournal of Magnetic Resonance
Volume272
DOIs
StatePublished - Nov 1 2016
Externally publishedYes

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Keywords

  • Constant time
  • J value
  • NMR spectroscopy
  • Scalar coupling
  • Structure elucidation

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Nuclear and High Energy Physics
  • Condensed Matter Physics

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