Measuring and mitigating inhibition during quantitative real time PCR analysis of viral nucleic acid extracts from large-volume environmental water samples

Engineering & Materials Science

• Nucleic acids 100%
• Water 36%
• RNA 27%
• Agricultural runoff 26%
• Pathogens 15%
• Sewage 14%
• Potable water 14%
• Density (specific gravity) 13%
• Groundwater 13%
• Viruses 13%
• Dilution 12%

Chemical Compounds

• Nucleic Acid 57%
• Specific Volume 43%
• Volume 39%
• Surface Water 35%
• Drinking Water 35%
• Dilution 27%
• Time 24%

Earth & Environmental Sciences

• measuring 45%
• water 20%
• RNA 20%
• analysis 18%
• agricultural runoff 13%
• virus 9%
• inhibitor 9%
• pathogen 9%
• dilution 8%
• sewage 8%
• drinking water 7%
• surface water 6%
• method 6%
• groundwater 5%
• effect 2%