Measurements and a model of the outer hair cell hydraulic conductivity

J. Tilak Ratnanather; Man Zhi; William E. Brownell; Aleksander S. Popel

doi:10.1016/0378-5955(96)00014-7

Measurements and a model of the outer hair cell hydraulic conductivity

J. Tilak Ratnanather, Man Zhi, William E. Brownell, Aleksander S. Popel

School of Medicine

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

The hydraulic conductivity of the cochlear outer hair cell (OHC) is central to the maintenance of the positive intracellular pressure necessary for its function as the cochlear amplifier. A mathematical model of osmotic water transport across the OHC membrane is formulated. The model relates the OHC hydraulic conductivity, L(p), to the rate of volume change in response to osmotic stimuli. L(p) is evaluated from osmotic experiments in which isolated OHCs are exposed to an hypotonic solution. The rate of volume increase in response to the hypotonic challenge was determined by a morphometric analysis of video images of cells. L(p) was found to be about 10^-14 m s^-1 Pa^-1 or equivalently, P(f) ~ 10^-4 cm s^-1. This is on the low side of values reported for different lipid bilayers and is 2 orders of magnitude lower than the hydraulic conductivity of red blood cells. The relation of the low OHC hydraulic conductivity to the composition and morphology of its membranes is discussed.

Original language	English (US)
Pages (from-to)	33-40
Number of pages	8
Journal	Hearing Research
Volume	96
Issue number	1-2
DOIs	https://doi.org/10.1016/0378-5955(96)00014-7
State	Published - Jul 1996

Keywords

Hydraulic skeleton
Mathematical model
Osmosis

ASJC Scopus subject areas

Sensory Systems

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10.1016/0378-5955(96)00014-7

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title = "Measurements and a model of the outer hair cell hydraulic conductivity",

abstract = "The hydraulic conductivity of the cochlear outer hair cell (OHC) is central to the maintenance of the positive intracellular pressure necessary for its function as the cochlear amplifier. A mathematical model of osmotic water transport across the OHC membrane is formulated. The model relates the OHC hydraulic conductivity, L(p), to the rate of volume change in response to osmotic stimuli. L(p) is evaluated from osmotic experiments in which isolated OHCs are exposed to an hypotonic solution. The rate of volume increase in response to the hypotonic challenge was determined by a morphometric analysis of video images of cells. L(p) was found to be about 10-14 m s-1 Pa-1 or equivalently, P(f) ~ 10-4 cm s-1. This is on the low side of values reported for different lipid bilayers and is 2 orders of magnitude lower than the hydraulic conductivity of red blood cells. The relation of the low OHC hydraulic conductivity to the composition and morphology of its membranes is discussed.",

keywords = "Hydraulic skeleton, Mathematical model, Osmosis",

author = "Ratnanather, {J. Tilak} and Man Zhi and Brownell, {William E.} and Popel, {Aleksander S.}",

note = "Funding Information: We thank Dr. M.E. Chertoff, I. Ghoshtagore and J. Crist for providing the tapes for analysis; C. Lee for assisting in the data acquisition; Drs. V.H. Huxley, R.A. Jerry, A.A. Spector and the reviewers for their critical comments. This work was supported by a DRF grant to J.T.R. and NIDCD Grant RO1-DC00354 to W.E.B. This material is based upon work supported by a Fellowship (to J.T.R.) from the Program in Mathematics and Molecular Biology at the University of California at Berkeley, which is supported by the National Science Foundation under Grant DMS-9406348. The Government has certain rights in this material•",

year = "1996",

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doi = "10.1016/0378-5955(96)00014-7",

language = "English (US)",

volume = "96",

pages = "33--40",

journal = "Hearing Research",

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TY - JOUR

T1 - Measurements and a model of the outer hair cell hydraulic conductivity

AU - Ratnanather, J. Tilak

AU - Zhi, Man

AU - Brownell, William E.

AU - Popel, Aleksander S.

N1 - Funding Information: We thank Dr. M.E. Chertoff, I. Ghoshtagore and J. Crist for providing the tapes for analysis; C. Lee for assisting in the data acquisition; Drs. V.H. Huxley, R.A. Jerry, A.A. Spector and the reviewers for their critical comments. This work was supported by a DRF grant to J.T.R. and NIDCD Grant RO1-DC00354 to W.E.B. This material is based upon work supported by a Fellowship (to J.T.R.) from the Program in Mathematics and Molecular Biology at the University of California at Berkeley, which is supported by the National Science Foundation under Grant DMS-9406348. The Government has certain rights in this material•

PY - 1996/7

Y1 - 1996/7

N2 - The hydraulic conductivity of the cochlear outer hair cell (OHC) is central to the maintenance of the positive intracellular pressure necessary for its function as the cochlear amplifier. A mathematical model of osmotic water transport across the OHC membrane is formulated. The model relates the OHC hydraulic conductivity, L(p), to the rate of volume change in response to osmotic stimuli. L(p) is evaluated from osmotic experiments in which isolated OHCs are exposed to an hypotonic solution. The rate of volume increase in response to the hypotonic challenge was determined by a morphometric analysis of video images of cells. L(p) was found to be about 10-14 m s-1 Pa-1 or equivalently, P(f) ~ 10-4 cm s-1. This is on the low side of values reported for different lipid bilayers and is 2 orders of magnitude lower than the hydraulic conductivity of red blood cells. The relation of the low OHC hydraulic conductivity to the composition and morphology of its membranes is discussed.

AB - The hydraulic conductivity of the cochlear outer hair cell (OHC) is central to the maintenance of the positive intracellular pressure necessary for its function as the cochlear amplifier. A mathematical model of osmotic water transport across the OHC membrane is formulated. The model relates the OHC hydraulic conductivity, L(p), to the rate of volume change in response to osmotic stimuli. L(p) is evaluated from osmotic experiments in which isolated OHCs are exposed to an hypotonic solution. The rate of volume increase in response to the hypotonic challenge was determined by a morphometric analysis of video images of cells. L(p) was found to be about 10-14 m s-1 Pa-1 or equivalently, P(f) ~ 10-4 cm s-1. This is on the low side of values reported for different lipid bilayers and is 2 orders of magnitude lower than the hydraulic conductivity of red blood cells. The relation of the low OHC hydraulic conductivity to the composition and morphology of its membranes is discussed.

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KW - Mathematical model

KW - Osmosis

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Measurements and a model of the outer hair cell hydraulic conductivity

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