Measurement of the phospholipase activity of endothelial lipase in mouse plasma

Debapriya Basu, Xia Lei, Joby Josekutty, M. Mahmood Hussain, Weijun Jin

Research output: Contribution to journalArticlepeer-review

Abstract

Endothelial lipase (EL) is a major negative regulator of plasma HDL levels in mice, rabbits, and most probably, humans. Although this regulatory function is critically dependent on EL's hydrolysis of HDL phospholipids, as yet there is no phospholipase assay specific for EL in plasma. We developed such an assay for the mouse enzyme using a commercially available phospholipid-like fluorescent substrate in combination with an EL neutralizing antibody. The specificity of the assay was established using EL knockout mice and its utility demonstrated by detection of an increase in plasma EL phospholipase activity following exposure of wild-type mice to lipopolysaccharide. The assay revealed that murine pre-heparin plasma does not contain measurable EL activity, indicating that the hydrolysis of HDL phospholipids by EL in vivo likely occurs on the cell surface.

Original languageEnglish (US)
Pages (from-to)282-289
Number of pages8
JournalJournal of Lipid Research
Volume54
Issue number1
DOIs
StatePublished - Jan 2013

Keywords

  • EL plasma
  • Heparin
  • Liposome

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

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