TY - JOUR
T1 - Measurement of spin-lattice relaxation times and kinetic rate constants in rat muscle using progressive partial saturation and steady-state saturation transfer
AU - Horská, Alena
AU - Spencer, Richard G.S.
PY - 1996/8
Y1 - 1996/8
N2 - 31P spin-lattice relaxation times (T1) of metabolites in rat calf muscle at 1.9 Tesla and the forward rate through the creatine kinase (CK) reaction have been measured using a new method based on modeling progressive saturation explicitly incorporating the effect of chemical exchange. In a separate series of experiments, we compared our method with inversion recovery both in vitro and in vivo, finding agreement between the techniques. We found that the T1 values of phosphocreatine (PCr) (6.6 ± 0.3 s), γ-ATP (2.6 ± 0.6 s), α-ATP (2.4 ± 0.4 s) and β-ATP (2.2 ± 0.2 s) are unchanged by stimulation of sufficient intensity to induce a 32% drop in PCr level. The errors in T1 values which arise when chemical exchange is neglected are calculated. These are found to be on the order of 20% for PCr and 30-50% for γ-ATP under typical conditions. Use of longer repetition times results in larger errors in measured values of T1. This source of error can be effectively eliminated by use of sufficiently short repetition times. We found that the rate constant of the forward CK reaction was increased 60% by stimulation, from 0.20 ± 0.03 s-1 to 0.32 ± 0.05 s- 1, but that the phosphorus flux did not change.
AB - 31P spin-lattice relaxation times (T1) of metabolites in rat calf muscle at 1.9 Tesla and the forward rate through the creatine kinase (CK) reaction have been measured using a new method based on modeling progressive saturation explicitly incorporating the effect of chemical exchange. In a separate series of experiments, we compared our method with inversion recovery both in vitro and in vivo, finding agreement between the techniques. We found that the T1 values of phosphocreatine (PCr) (6.6 ± 0.3 s), γ-ATP (2.6 ± 0.6 s), α-ATP (2.4 ± 0.4 s) and β-ATP (2.2 ± 0.2 s) are unchanged by stimulation of sufficient intensity to induce a 32% drop in PCr level. The errors in T1 values which arise when chemical exchange is neglected are calculated. These are found to be on the order of 20% for PCr and 30-50% for γ-ATP under typical conditions. Use of longer repetition times results in larger errors in measured values of T1. This source of error can be effectively eliminated by use of sufficiently short repetition times. We found that the rate constant of the forward CK reaction was increased 60% by stimulation, from 0.20 ± 0.03 s-1 to 0.32 ± 0.05 s- 1, but that the phosphorus flux did not change.
KW - NMR
KW - creatine kinase
KW - muscle
KW - spin-lattice relaxation time
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U2 - 10.1002/mrm.1910360210
DO - 10.1002/mrm.1910360210
M3 - Article
C2 - 8843377
AN - SCOPUS:0030203305
SN - 0740-3194
VL - 36
SP - 232
EP - 240
JO - Magnetic resonance in medicine
JF - Magnetic resonance in medicine
IS - 2
ER -