Measurement of plasma dihydrotestosterone by competitive protein-binding analysis

Roland R. Tremblay, Inese Z. Beitins, Avinoam Kowarski, Claude J. Migeon

Research output: Contribution to journalArticle

Abstract

A competitive protein binding method for the measurement of dihydrotestosterone (DHT) concentration in plasma is described. The mean recovery throughout the procedure was 75 ± 11%. Water blanks (10 ml) ranged from 0 to 0.2 ng per sample. The precision and accuracy were good. The specificity was checked by preparation of two further derivatives of DHT. Mean plasma values of DHT for normal adult women and men were respectively 21.7 ± 4.3 (S.D.) and 50.1 ± 14.4 (S.D.) ng/100 ml. Simultaneously by taking a smaller aliquot following the paper chromatography, plasma testosterone (T) concentrations could be measured by competitive protein binding.

Original languageEnglish (US)
Pages (from-to)29-40
Number of pages12
JournalSteroids
Volume16
Issue numberC
DOIs
StatePublished - Jan 1 1970

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology
  • Pharmacology
  • Clinical Biochemistry
  • Organic Chemistry

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