Measurement of plasma aldosterone by radioimmunoassay

Francis Bayard, Inese Z. Beitins, Avinoam Kowarski, Claude J. Migeon

Research output: Contribution to journalArticle

Abstract

A radioimmunoassay for the measurement of plasma aldosterone is described. Aldosterone-3-carboxymethoxime-18,21-diacetate was coupled with rabbit albumin and antibodies produced in rabbits. Five to 20 ml of plasma, with l,2-3H-aldosterone added for recovery, was extracted and purification achieved by one paper chromatography. After 20 min incubation of the antibody-steroid mixture at 37 C, bound and free fractions were separated using Florisil. The mean recovery of 3H-aldosterone, after extraction and chromatography, was 66.3 ± 13.6 (sd)%. The specificity of the method was confirmed by comparing the results obtained with those of the double isotope derivative method on the same plasma samples. The nonspecific blank was 0.088 ± 0.055 ng/sample (mean ±sd). A linear relationship between amounts of aldosterone measured and the size of the samples confirmed accuracy. Coefficients of variation of 14, 13.2 and 10.5% were obtained at levels of 4, 18 and 51 ng of aldosterone/100 ml of plasma. Results on normal individuals were comparable to those in the literature.

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
JournalJournal of Clinical Endocrinology and Metabolism
Volume31
Issue number1
DOIs
StatePublished - Jan 1 1970

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

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