Measurement of mycophenolate mofetil effect in transplant recipients

Nobuji Ogawa, Naoki Nagashima, Michio Nakamura, Ahmed Shalabi, Warren R. Maley, James F. Burdick

Research output: Contribution to journalArticle

Abstract

Background. Immunosuppression involves the nature of the immunosuppressive agents and individual differences in patient factors. We investigated whether the effect of mycophenolate mofetil (MMF) is measurable using an in vitro measure of immunocompetence and related its effects to cyclosporine (CsA) in vitro. Methods. Liver or kidney transplant recipients receiving prednisone; CsA or tacrolimus; and MMF, azathioprine (AZA), or neither, were studied. Immunocompetence was assessed by one-way mixed lymphocyte culture using patients' peripheral blood leukocytes (PBL) and three validated stimulators. The effect of immunosuppressive agents added in vitro on normal PBL stimulation by Staphylococcus enterotoxin B was determined by the carboxyfluorescein diacetate succinimidyl ester measurement of division. Results. Patients receiving MMF had an average immunocompetence level of 12±23, compared with 39.7±65 and 25.5±42 for those receiving AZA or neither AZA nor MMF, respectively. Thus, there was an approximately 80% suppression of the response in the MMF group. Assessment of normal cell division revealed that CsA allowed multiple cell generations but suppressed the numbers of cells in each, whereas MMF blocked proliferation into subsequent generations. Addition of clinically relevant levels of mycophenolic acid, the active agent for MMF, added to more moderate levels of CsA, was required to achieve greater than 80% suppression, consistent with the degree of immunocompetence depression measured in patients. Conclusions. These data provide the novel finding that the effect of MMF treatment on patients is measurable in their PBL as decreased immunocompetence in vitro. The effect of MMF on normal PBL approximates the 80% inhibition that we found in patients. Moreover, the effect of MMF on cell division provides a rationale for the superior effectiveness of regimens including MMF.

Original languageEnglish (US)
Pages (from-to)422-427
Number of pages6
JournalTransplantation
Volume72
Issue number3
StatePublished - Aug 15 2001

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Mycophenolic Acid
Immunocompetence
Azathioprine
Leukocytes
Immunosuppressive Agents
Cell Division
Transplant Recipients
Tacrolimus
Prednisone
Individuality
Immunosuppression
Cyclosporine

ASJC Scopus subject areas

  • Transplantation
  • Immunology

Cite this

Ogawa, N., Nagashima, N., Nakamura, M., Shalabi, A., Maley, W. R., & Burdick, J. F. (2001). Measurement of mycophenolate mofetil effect in transplant recipients. Transplantation, 72(3), 422-427.

Measurement of mycophenolate mofetil effect in transplant recipients. / Ogawa, Nobuji; Nagashima, Naoki; Nakamura, Michio; Shalabi, Ahmed; Maley, Warren R.; Burdick, James F.

In: Transplantation, Vol. 72, No. 3, 15.08.2001, p. 422-427.

Research output: Contribution to journalArticle

Ogawa, N, Nagashima, N, Nakamura, M, Shalabi, A, Maley, WR & Burdick, JF 2001, 'Measurement of mycophenolate mofetil effect in transplant recipients', Transplantation, vol. 72, no. 3, pp. 422-427.
Ogawa N, Nagashima N, Nakamura M, Shalabi A, Maley WR, Burdick JF. Measurement of mycophenolate mofetil effect in transplant recipients. Transplantation. 2001 Aug 15;72(3):422-427.
Ogawa, Nobuji ; Nagashima, Naoki ; Nakamura, Michio ; Shalabi, Ahmed ; Maley, Warren R. ; Burdick, James F. / Measurement of mycophenolate mofetil effect in transplant recipients. In: Transplantation. 2001 ; Vol. 72, No. 3. pp. 422-427.
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abstract = "Background. Immunosuppression involves the nature of the immunosuppressive agents and individual differences in patient factors. We investigated whether the effect of mycophenolate mofetil (MMF) is measurable using an in vitro measure of immunocompetence and related its effects to cyclosporine (CsA) in vitro. Methods. Liver or kidney transplant recipients receiving prednisone; CsA or tacrolimus; and MMF, azathioprine (AZA), or neither, were studied. Immunocompetence was assessed by one-way mixed lymphocyte culture using patients' peripheral blood leukocytes (PBL) and three validated stimulators. The effect of immunosuppressive agents added in vitro on normal PBL stimulation by Staphylococcus enterotoxin B was determined by the carboxyfluorescein diacetate succinimidyl ester measurement of division. Results. Patients receiving MMF had an average immunocompetence level of 12±23, compared with 39.7±65 and 25.5±42 for those receiving AZA or neither AZA nor MMF, respectively. Thus, there was an approximately 80{\%} suppression of the response in the MMF group. Assessment of normal cell division revealed that CsA allowed multiple cell generations but suppressed the numbers of cells in each, whereas MMF blocked proliferation into subsequent generations. Addition of clinically relevant levels of mycophenolic acid, the active agent for MMF, added to more moderate levels of CsA, was required to achieve greater than 80{\%} suppression, consistent with the degree of immunocompetence depression measured in patients. Conclusions. These data provide the novel finding that the effect of MMF treatment on patients is measurable in their PBL as decreased immunocompetence in vitro. The effect of MMF on normal PBL approximates the 80{\%} inhibition that we found in patients. Moreover, the effect of MMF on cell division provides a rationale for the superior effectiveness of regimens including MMF.",
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