Measurement of membrane protein lateral diffusion in single cells

M. Edidin; Y. Zagyansky; T. J. Lardner

doi:10.1126/science.1246629

Measurement of membrane protein lateral diffusion in single cells

M. Edidin, Y. Zagyansky, T. J. Lardner

Krieger School of Arts and Sciences

Research output: Contribution to journal › Article › peer-review

207 Scopus citations

Abstract

Fluorescence rapidly returns to spots bleached by a laser beam in the continuous fluorescence of cultured cells labeled on the surface with fluorescein isothiocyanate. The rate of recovery of fluorescence after bleaching can be interpreted as a measure of the lateral diffusion of integral membrane proteins labeled with fluorescein.

Original language	English (US)
Pages (from-to)	466-468
Number of pages	3
Journal	Science
Volume	191
Issue number	4226
DOIs	https://doi.org/10.1126/science.1246629
State	Published - 1976

ASJC Scopus subject areas

General

Access to Document

10.1126/science.1246629

Cite this

@article{4a70175ab2c44d41a93f246296380469,

title = "Measurement of membrane protein lateral diffusion in single cells",

abstract = "Fluorescence rapidly returns to spots bleached by a laser beam in the continuous fluorescence of cultured cells labeled on the surface with fluorescein isothiocyanate. The rate of recovery of fluorescence after bleaching can be interpreted as a measure of the lateral diffusion of integral membrane proteins labeled with fluorescein.",

author = "M. Edidin and Y. Zagyansky and Lardner, {T. J.}",

year = "1976",

doi = "10.1126/science.1246629",

language = "English (US)",

volume = "191",

pages = "466--468",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "4226",

}

TY - JOUR

T1 - Measurement of membrane protein lateral diffusion in single cells

AU - Edidin, M.

AU - Zagyansky, Y.

AU - Lardner, T. J.

PY - 1976

Y1 - 1976

N2 - Fluorescence rapidly returns to spots bleached by a laser beam in the continuous fluorescence of cultured cells labeled on the surface with fluorescein isothiocyanate. The rate of recovery of fluorescence after bleaching can be interpreted as a measure of the lateral diffusion of integral membrane proteins labeled with fluorescein.

AB - Fluorescence rapidly returns to spots bleached by a laser beam in the continuous fluorescence of cultured cells labeled on the surface with fluorescein isothiocyanate. The rate of recovery of fluorescence after bleaching can be interpreted as a measure of the lateral diffusion of integral membrane proteins labeled with fluorescein.

UR - http://www.scopus.com/inward/record.url?scp=0017236539&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017236539&partnerID=8YFLogxK

U2 - 10.1126/science.1246629

DO - 10.1126/science.1246629

M3 - Article

C2 - 1246629

AN - SCOPUS:0017236539

SN - 0036-8075

VL - 191

SP - 466

EP - 468

JO - Science

JF - Science

IS - 4226

ER -

Measurement of membrane protein lateral diffusion in single cells

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this