Measurement of low picomolar levels of triamcinolone acetonide in human bronchoalveolar lavage fluid by gas chromatography-electron-capture negative-ion mass spectrometry

Walter C. Hubbard, Mark Chang Hwa Liu, Carol Bickel, Domenick Argenti, Don Heald, Robert P. Schleimer

Research output: Contribution to journalArticle

Abstract

The intense inherent electron-capture properties of the C21 acetate derivative of triamcinolone acetonide (TAA) under methane chemical ionization mass spectrometric conditions were exploited for the development of a highly sensitive and selective gas chromatography-mass spectrometric (GC-MS) technique for measurement of levels of TAA in human bronchoalveolar lavage (BAL) fluid. After the addition of 3.0 ng of a heptadeuterated analog of TAA and varying concentrations of TAA to 2-ml aliquots of BAL fluid, the deuterium and protium forms of the steroid were extracted with diethyl ether, converted to the C21 acetate derivative, and purified via adsorptive chromatography prior to GC-MS analysis. Standard curves obtained from 2-ml aliquots of BAL fluid were linear over a wide range of concentrations of TAA from 0.0 to 24,600 pg/2-ml aliquots of BAL fluid. Levels as low as 6.0 pg/ml (13.8 pmol L-1) in BAL fluid can be reliably determined in 2-ml aliquots of the biological fluid with

Original languageEnglish (US)
Pages (from-to)18-25
Number of pages8
JournalAnalytical Biochemistry
Volume290
Issue number1
DOIs
StatePublished - Mar 1 2001

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Triamcinolone Acetonide
Bronchoalveolar Lavage Fluid
Gas chromatography
Gas Chromatography
Mass spectrometry
Mass Spectrometry
Negative ions
Electrons
Ions
Fluids
Acetates
Deuterium
Derivatives
Methane
Ether
Chromatography
Ionization
Steroids

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Measurement of low picomolar levels of triamcinolone acetonide in human bronchoalveolar lavage fluid by gas chromatography-electron-capture negative-ion mass spectrometry. / Hubbard, Walter C.; Liu, Mark Chang Hwa; Bickel, Carol; Argenti, Domenick; Heald, Don; Schleimer, Robert P.

In: Analytical Biochemistry, Vol. 290, No. 1, 01.03.2001, p. 18-25.

Research output: Contribution to journalArticle

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