Measurement of IgG blocking antibody in human serum: Comparison of ELISA with monoclonal antibody and fluorogenic substrate and Staphylococcus protein A solid-phase RIA

Ha Baik Lee, N Franklin Adkinson

Research output: Contribution to journalArticle

Abstract

We compared ELISA with mouse monoclonal antihuman γ-chain antibody and a fluorogenic substrate with the Staphylococcus protein A solid-phase radioimmunoassay (SPRIA) in the measurement of specific IgG antibody to short ragweed pollen. Sera from 51 ragweed-allergic patients undergoing allergen immunotherapy were evaluated for ragweed-specific IgG antibodies with the same ragweed extract in the two assay systems. With optimal conditions, the ELISA and SPRIA displayed comparable positive thresholds (~1 ng/ml of ragweed-specific IgG). Both assays also demonstrated consistently parallel dilution curves with 51 sera (mean interdilutional coefficient of variation [CV] 80%) sera. Occasionally, discrepant results would not be expected to be of clinical significance. Performance specifications for the ragweed IgC ELISA were acceptable, and the technical ease of ELISA is superior to the labor-intensive SPRIA. For routine clinical use, the ragweed ELISA is a suitable alternative to SPRIA.

Original languageEnglish (US)
Pages (from-to)11-19
Number of pages9
JournalThe Journal of Allergy and Clinical Immunology
Volume82
Issue number1
DOIs
StatePublished - 1988

Fingerprint

Ambrosia
Blocking Antibodies
Staphylococcal Protein A
Staphylococcus
Fluorescent Dyes
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
Radioimmunoassay
Serum
Antibodies
Immunologic Desensitization

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

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title = "Measurement of IgG blocking antibody in human serum: Comparison of ELISA with monoclonal antibody and fluorogenic substrate and Staphylococcus protein A solid-phase RIA",
abstract = "We compared ELISA with mouse monoclonal antihuman γ-chain antibody and a fluorogenic substrate with the Staphylococcus protein A solid-phase radioimmunoassay (SPRIA) in the measurement of specific IgG antibody to short ragweed pollen. Sera from 51 ragweed-allergic patients undergoing allergen immunotherapy were evaluated for ragweed-specific IgG antibodies with the same ragweed extract in the two assay systems. With optimal conditions, the ELISA and SPRIA displayed comparable positive thresholds (~1 ng/ml of ragweed-specific IgG). Both assays also demonstrated consistently parallel dilution curves with 51 sera (mean interdilutional coefficient of variation [CV] 80{\%}) sera. Occasionally, discrepant results would not be expected to be of clinical significance. Performance specifications for the ragweed IgC ELISA were acceptable, and the technical ease of ELISA is superior to the labor-intensive SPRIA. For routine clinical use, the ragweed ELISA is a suitable alternative to SPRIA.",
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N2 - We compared ELISA with mouse monoclonal antihuman γ-chain antibody and a fluorogenic substrate with the Staphylococcus protein A solid-phase radioimmunoassay (SPRIA) in the measurement of specific IgG antibody to short ragweed pollen. Sera from 51 ragweed-allergic patients undergoing allergen immunotherapy were evaluated for ragweed-specific IgG antibodies with the same ragweed extract in the two assay systems. With optimal conditions, the ELISA and SPRIA displayed comparable positive thresholds (~1 ng/ml of ragweed-specific IgG). Both assays also demonstrated consistently parallel dilution curves with 51 sera (mean interdilutional coefficient of variation [CV] 80%) sera. Occasionally, discrepant results would not be expected to be of clinical significance. Performance specifications for the ragweed IgC ELISA were acceptable, and the technical ease of ELISA is superior to the labor-intensive SPRIA. For routine clinical use, the ragweed ELISA is a suitable alternative to SPRIA.

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