We compared ELISA with mouse monoclonal antihuman γ-chain antibody and a fluorogenic substrate with the Staphylococcus protein A solid-phase radioimmunoassay (SPRIA) in the measurement of specific IgG antibody to short ragweed pollen. Sera from 51 ragweed-allergic patients undergoing allergen immunotherapy were evaluated for ragweed-specific IgG antibodies with the same ragweed extract in the two assay systems. With optimal conditions, the ELISA and SPRIA displayed comparable positive thresholds (~1 ng/ml of ragweed-specific IgG). Both assays also demonstrated consistently parallel dilution curves with 51 sera (mean interdilutional coefficient of variation [CV] 80%) sera. Occasionally, discrepant results would not be expected to be of clinical significance. Performance specifications for the ragweed IgC ELISA were acceptable, and the technical ease of ELISA is superior to the labor-intensive SPRIA. For routine clinical use, the ragweed ELISA is a suitable alternative to SPRIA.
ASJC Scopus subject areas
- Immunology and Allergy