Abstract
The peptide N-Acetylaspartylglutamate (NAAG) is hydrolyzed by N-Acetylated-alpha-linked-acidic dipeptidase (NAALADase, glutamate carboxypeptidase II) into N-Acetylated aspartate (NAA) and glutamate. Hydrolysis can be measured as described in this unit by employing radiolabeled NAAG (NAA-[(3)H]glu) as the substrate. The occurrence of NAALADase activity in a wide range of tissues has implications for a variety of physiological purposes. The assay described here is useful for the analysis of NAALADase activity and its inhibition in brain synaptosomal preparations, tissue homogenates and tissue culture cell pellets.
| Original language | English (US) |
|---|---|
| Journal | Current protocols in pharmacology / editorial board, S.J. Enna (editor-in-chief) ... [et al.] |
| Volume | Chapter 3 |
| State | Published - Feb 2002 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Medicine(all)
Cite this
Measurement of glutamate carboxypeptidase II (NAALADase) enzyme activity by the hydrolysis of [ 3H]-N-acetylaspartylglutamate (NAAG). / Tiffany, Carol W; Slusher, Barbara.
In: Current protocols in pharmacology / editorial board, S.J. Enna (editor-in-chief) ... [et al.], Vol. Chapter 3, 02.2002.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Measurement of glutamate carboxypeptidase II (NAALADase) enzyme activity by the hydrolysis of [ 3H]-N-acetylaspartylglutamate (NAAG).
AU - Tiffany, Carol W
AU - Slusher, Barbara
PY - 2002/2
Y1 - 2002/2
N2 - The peptide N-Acetylaspartylglutamate (NAAG) is hydrolyzed by N-Acetylated-alpha-linked-acidic dipeptidase (NAALADase, glutamate carboxypeptidase II) into N-Acetylated aspartate (NAA) and glutamate. Hydrolysis can be measured as described in this unit by employing radiolabeled NAAG (NAA-[(3)H]glu) as the substrate. The occurrence of NAALADase activity in a wide range of tissues has implications for a variety of physiological purposes. The assay described here is useful for the analysis of NAALADase activity and its inhibition in brain synaptosomal preparations, tissue homogenates and tissue culture cell pellets.
AB - The peptide N-Acetylaspartylglutamate (NAAG) is hydrolyzed by N-Acetylated-alpha-linked-acidic dipeptidase (NAALADase, glutamate carboxypeptidase II) into N-Acetylated aspartate (NAA) and glutamate. Hydrolysis can be measured as described in this unit by employing radiolabeled NAAG (NAA-[(3)H]glu) as the substrate. The occurrence of NAALADase activity in a wide range of tissues has implications for a variety of physiological purposes. The assay described here is useful for the analysis of NAALADase activity and its inhibition in brain synaptosomal preparations, tissue homogenates and tissue culture cell pellets.
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M3 - Article
C2 - 21959764
AN - SCOPUS:84857392621
VL - Chapter 3
JO - Current Protocols in Pharmacology
JF - Current Protocols in Pharmacology
SN - 1934-8282
ER -