TY - JOUR
T1 - Measurement of flow-mediated dilation of mouse femoral artery in vivo by optical coherence tomography
AU - Song, Weiye
AU - Zhou, Libo
AU - Kot, Kevin L.
AU - Fan, Huijie
AU - Han, Jingyan
AU - Yi, Ji
N1 - Funding Information:
This study is supported by Evans medical foundation (to J.Y.), BU CTSI KL2TR001411 (to J.Y.), American Heart Association 14SDG20140036 (to J.H.) and National Institutes of Health R56HL130194 (to J.H.)
Publisher Copyright:
© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2018/11
Y1 - 2018/11
N2 - Flow-mediated vasodilation (FMD) is used for assessment of vascular endothelial function in humans as a predictor of cardiovascular events. It has been challenging to carry it on preclinical murine models due to the diminutive size of the femoral artery. Here, we present a new approach to accurately measure the blood velocity and femoral artery diameters of mice by acquiring Doppler optical coherence tomography and optical coherence tomography angiography continuously within 1 single experimental scanning protocol. Using the 3-dimensional imaging and new velocity algorithm, the measurement precision of diameter, blood flow, velocity and wall shear stress are improved to 0.91%, 11.0%, 10.7% and 14.0%, respectively. FMD of healthy mouse femoral artery measured by this method was 11.96% ± 0.98%, which was blunted to 5.69% ± 0.4% by intravenous administration of endothelial nitric oxide synthase inhibitor (L-NG-Nitroarginine methyl ester), in agreement with that reported in the literature.
AB - Flow-mediated vasodilation (FMD) is used for assessment of vascular endothelial function in humans as a predictor of cardiovascular events. It has been challenging to carry it on preclinical murine models due to the diminutive size of the femoral artery. Here, we present a new approach to accurately measure the blood velocity and femoral artery diameters of mice by acquiring Doppler optical coherence tomography and optical coherence tomography angiography continuously within 1 single experimental scanning protocol. Using the 3-dimensional imaging and new velocity algorithm, the measurement precision of diameter, blood flow, velocity and wall shear stress are improved to 0.91%, 11.0%, 10.7% and 14.0%, respectively. FMD of healthy mouse femoral artery measured by this method was 11.96% ± 0.98%, which was blunted to 5.69% ± 0.4% by intravenous administration of endothelial nitric oxide synthase inhibitor (L-NG-Nitroarginine methyl ester), in agreement with that reported in the literature.
KW - arterial wall shear stress
KW - flow-mediated dilation
KW - mouse femoral artery
KW - optical coherence tomography
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U2 - 10.1002/jbio.201800053
DO - 10.1002/jbio.201800053
M3 - Article
C2 - 29855165
AN - SCOPUS:85056231501
SN - 1864-063X
VL - 11
JO - Journal of Biophotonics
JF - Journal of Biophotonics
IS - 11
M1 - e201800053
ER -