Measurement of cytosolic calcium using 19F NMR

E. Murphy; L. Levy; B. Raju; C. Steenbergen; J. T. Gerig; P. Singh; R. E. London

doi:10.1289/ehp.908495

Measurement of cytosolic calcium using ¹⁹F NMR

E. Murphy, L. Levy, B. Raju, C. Steenbergen, J. T. Gerig, P. Singh, R. E. London

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Fluorine-19 nuclear magnetic resonance (NMR) studies of cells and perfused organs loaded with fluorinated ion chelators represent a new approach to determining cytosolic free calcium levels in situ. The molecular basis for this approach and the relative advantages and disadvantages of the NMR technique are discussed in this paper. Results obtained on perfused normoxic and ischemic rat hearts are presented, indicating that ischemia is associated with an elevation in the level of cytosolic free calcium before the onset of irreversible cell injury. The results are therefore consistent with this elevation playing a causative role in the mediation of myocardial cell injury resulting from ischemia.

Original language	English (US)
Pages (from-to)	95-98
Number of pages	4
Journal	Environmental health perspectives
Volume	84
DOIs	https://doi.org/10.1289/ehp.908495
State	Published - 1990
Externally published	Yes

ASJC Scopus subject areas

Public Health, Environmental and Occupational Health
Health, Toxicology and Mutagenesis

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title = "Measurement of cytosolic calcium using 19F NMR",

abstract = "Fluorine-19 nuclear magnetic resonance (NMR) studies of cells and perfused organs loaded with fluorinated ion chelators represent a new approach to determining cytosolic free calcium levels in situ. The molecular basis for this approach and the relative advantages and disadvantages of the NMR technique are discussed in this paper. Results obtained on perfused normoxic and ischemic rat hearts are presented, indicating that ischemia is associated with an elevation in the level of cytosolic free calcium before the onset of irreversible cell injury. The results are therefore consistent with this elevation playing a causative role in the mediation of myocardial cell injury resulting from ischemia.",

author = "E. Murphy and L. Levy and B. Raju and C. Steenbergen and Gerig, {J. T.} and P. Singh and London, {R. E.}",

year = "1990",

doi = "10.1289/ehp.908495",

language = "English (US)",

volume = "84",

pages = "95--98",

journal = "Environmental health perspectives",

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T1 - Measurement of cytosolic calcium using 19F NMR

AU - Murphy, E.

AU - Levy, L.

AU - Raju, B.

AU - Steenbergen, C.

AU - Gerig, J. T.

AU - Singh, P.

AU - London, R. E.

PY - 1990

Y1 - 1990

N2 - Fluorine-19 nuclear magnetic resonance (NMR) studies of cells and perfused organs loaded with fluorinated ion chelators represent a new approach to determining cytosolic free calcium levels in situ. The molecular basis for this approach and the relative advantages and disadvantages of the NMR technique are discussed in this paper. Results obtained on perfused normoxic and ischemic rat hearts are presented, indicating that ischemia is associated with an elevation in the level of cytosolic free calcium before the onset of irreversible cell injury. The results are therefore consistent with this elevation playing a causative role in the mediation of myocardial cell injury resulting from ischemia.

AB - Fluorine-19 nuclear magnetic resonance (NMR) studies of cells and perfused organs loaded with fluorinated ion chelators represent a new approach to determining cytosolic free calcium levels in situ. The molecular basis for this approach and the relative advantages and disadvantages of the NMR technique are discussed in this paper. Results obtained on perfused normoxic and ischemic rat hearts are presented, indicating that ischemia is associated with an elevation in the level of cytosolic free calcium before the onset of irreversible cell injury. The results are therefore consistent with this elevation playing a causative role in the mediation of myocardial cell injury resulting from ischemia.

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DO - 10.1289/ehp.908495

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AN - SCOPUS:0024997257

SN - 0091-6765

VL - 84

SP - 95

EP - 98

JO - Environmental health perspectives

JF - Environmental health perspectives

ER -

Measurement of cytosolic calcium using ¹⁹F NMR

Abstract

ASJC Scopus subject areas

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