Measurement of chromosomal aberrations, sister chromatid exchange, hprt mutations, and DNA adducts in peripheral lymphocytes of human populations at increased risk for cancer

D. Jacobson-Kram, R. J. Albertini, R. F. Branda, M. T. Falta, P. T. Iype, K. Kolodner, S. H. Liou, M. A. McDiarmid, M. Morris, J. A. Nicklas, J. P. O'Neill, M. C. Poirier, D. Putman, Paul Timothy Strickland, J. R. Williams, S. Xiao

Research output: Contribution to journalArticle

Abstract

Using a multidisciplinary approach, we have measured various indicators of DNA damage in peripheral lymphocytes of human populations potentially at increased risk for cancer. Sister chromatid exchanges (SCE) and polycyclic aromatic hydrocarbon (PAH)-DNA adducts were evaluated in a group of firefighters; chromosomal aberrations and hprt mutations were evaluated in a group of cancer patients undergoing radioimmunoglobulin therapy (RIT); SCE and acrolein-modified DNA were measured in cancer chemotherapy patients and in pharmacists preparing chemotherapy prescriptions; and SCE and PAH-DNA adducts are being measured in U.S. army troops stationed in Kuwait. Our results indicate that both SCE and PAH-DNA adduct levels were not elevated in firefighters, but that other factors such as smoking status and race were risk factors for increased SCE and PAH-DNA adducts. RIT was found to increase background rates of chromosome-type aberrations and frequencies of hprt mutations and there was a strong correlation between levels of therapy-induced chromosome damage sustained in vivo and in vitro sensitivity to radiation-induced chromosome damage. Peripheral blood lymphocytes of cancer patients treated with cyclophosphamide showed higher levels of SCE and had a higher incidence of acrolein adducts in DNA. Lymphocytes from pharmacists preparing antineoplastic drugs were found to acquire increased in vitro sensitivity to SCE induction by phosphoramide mustard with increased lifetime duration of drug handling. A prospective, longitudinal study was performed to identify environmental factors that modulate genetic damage in breast cancer patients. Women with benign breast masses and no apparent disease served as controls. Mutant frequency, cloning efficiency, and chromosomal aberration frequency did not differ significantly among the three groups. The results described and the data being gathered on troops stationed in Kuwait suggest that all the methodologies described can be useful in screening human populations for mutagenic exposures.

Original languageEnglish (US)
Pages (from-to)121-125
Number of pages5
JournalEnvironmental Health Perspectives
Volume101
Issue numberSUPPL. 3
StatePublished - 1993
Externally publishedYes

Fingerprint

Sister Chromatid Exchange
Lymphocytes
DNA Adducts
Aberrations
Chromosome Aberrations
mutation
cancer
Chromosomes
Acrolein
DNA
Mutation
Chemotherapy
PAH
Neoplasms
Firefighters
Kuwait
chemotherapy
chromosome
damage
Cloning

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Public Health, Environmental and Occupational Health

Cite this

Jacobson-Kram, D., Albertini, R. J., Branda, R. F., Falta, M. T., Iype, P. T., Kolodner, K., ... Xiao, S. (1993). Measurement of chromosomal aberrations, sister chromatid exchange, hprt mutations, and DNA adducts in peripheral lymphocytes of human populations at increased risk for cancer. Environmental Health Perspectives, 101(SUPPL. 3), 121-125.

Measurement of chromosomal aberrations, sister chromatid exchange, hprt mutations, and DNA adducts in peripheral lymphocytes of human populations at increased risk for cancer. / Jacobson-Kram, D.; Albertini, R. J.; Branda, R. F.; Falta, M. T.; Iype, P. T.; Kolodner, K.; Liou, S. H.; McDiarmid, M. A.; Morris, M.; Nicklas, J. A.; O'Neill, J. P.; Poirier, M. C.; Putman, D.; Strickland, Paul Timothy; Williams, J. R.; Xiao, S.

In: Environmental Health Perspectives, Vol. 101, No. SUPPL. 3, 1993, p. 121-125.

Research output: Contribution to journalArticle

Jacobson-Kram, D, Albertini, RJ, Branda, RF, Falta, MT, Iype, PT, Kolodner, K, Liou, SH, McDiarmid, MA, Morris, M, Nicklas, JA, O'Neill, JP, Poirier, MC, Putman, D, Strickland, PT, Williams, JR & Xiao, S 1993, 'Measurement of chromosomal aberrations, sister chromatid exchange, hprt mutations, and DNA adducts in peripheral lymphocytes of human populations at increased risk for cancer', Environmental Health Perspectives, vol. 101, no. SUPPL. 3, pp. 121-125.
Jacobson-Kram, D. ; Albertini, R. J. ; Branda, R. F. ; Falta, M. T. ; Iype, P. T. ; Kolodner, K. ; Liou, S. H. ; McDiarmid, M. A. ; Morris, M. ; Nicklas, J. A. ; O'Neill, J. P. ; Poirier, M. C. ; Putman, D. ; Strickland, Paul Timothy ; Williams, J. R. ; Xiao, S. / Measurement of chromosomal aberrations, sister chromatid exchange, hprt mutations, and DNA adducts in peripheral lymphocytes of human populations at increased risk for cancer. In: Environmental Health Perspectives. 1993 ; Vol. 101, No. SUPPL. 3. pp. 121-125.
@article{8d62ab330d9b4d33a458ee8ad7939b93,
title = "Measurement of chromosomal aberrations, sister chromatid exchange, hprt mutations, and DNA adducts in peripheral lymphocytes of human populations at increased risk for cancer",
abstract = "Using a multidisciplinary approach, we have measured various indicators of DNA damage in peripheral lymphocytes of human populations potentially at increased risk for cancer. Sister chromatid exchanges (SCE) and polycyclic aromatic hydrocarbon (PAH)-DNA adducts were evaluated in a group of firefighters; chromosomal aberrations and hprt mutations were evaluated in a group of cancer patients undergoing radioimmunoglobulin therapy (RIT); SCE and acrolein-modified DNA were measured in cancer chemotherapy patients and in pharmacists preparing chemotherapy prescriptions; and SCE and PAH-DNA adducts are being measured in U.S. army troops stationed in Kuwait. Our results indicate that both SCE and PAH-DNA adduct levels were not elevated in firefighters, but that other factors such as smoking status and race were risk factors for increased SCE and PAH-DNA adducts. RIT was found to increase background rates of chromosome-type aberrations and frequencies of hprt mutations and there was a strong correlation between levels of therapy-induced chromosome damage sustained in vivo and in vitro sensitivity to radiation-induced chromosome damage. Peripheral blood lymphocytes of cancer patients treated with cyclophosphamide showed higher levels of SCE and had a higher incidence of acrolein adducts in DNA. Lymphocytes from pharmacists preparing antineoplastic drugs were found to acquire increased in vitro sensitivity to SCE induction by phosphoramide mustard with increased lifetime duration of drug handling. A prospective, longitudinal study was performed to identify environmental factors that modulate genetic damage in breast cancer patients. Women with benign breast masses and no apparent disease served as controls. Mutant frequency, cloning efficiency, and chromosomal aberration frequency did not differ significantly among the three groups. The results described and the data being gathered on troops stationed in Kuwait suggest that all the methodologies described can be useful in screening human populations for mutagenic exposures.",
author = "D. Jacobson-Kram and Albertini, {R. J.} and Branda, {R. F.} and Falta, {M. T.} and Iype, {P. T.} and K. Kolodner and Liou, {S. H.} and McDiarmid, {M. A.} and M. Morris and Nicklas, {J. A.} and O'Neill, {J. P.} and Poirier, {M. C.} and D. Putman and Strickland, {Paul Timothy} and Williams, {J. R.} and S. Xiao",
year = "1993",
language = "English (US)",
volume = "101",
pages = "121--125",
journal = "Environmental Health Perspectives",
issn = "0091-6765",
publisher = "Public Health Services, US Dept of Health and Human Services",
number = "SUPPL. 3",

}

TY - JOUR

T1 - Measurement of chromosomal aberrations, sister chromatid exchange, hprt mutations, and DNA adducts in peripheral lymphocytes of human populations at increased risk for cancer

AU - Jacobson-Kram, D.

AU - Albertini, R. J.

AU - Branda, R. F.

AU - Falta, M. T.

AU - Iype, P. T.

AU - Kolodner, K.

AU - Liou, S. H.

AU - McDiarmid, M. A.

AU - Morris, M.

AU - Nicklas, J. A.

AU - O'Neill, J. P.

AU - Poirier, M. C.

AU - Putman, D.

AU - Strickland, Paul Timothy

AU - Williams, J. R.

AU - Xiao, S.

PY - 1993

Y1 - 1993

N2 - Using a multidisciplinary approach, we have measured various indicators of DNA damage in peripheral lymphocytes of human populations potentially at increased risk for cancer. Sister chromatid exchanges (SCE) and polycyclic aromatic hydrocarbon (PAH)-DNA adducts were evaluated in a group of firefighters; chromosomal aberrations and hprt mutations were evaluated in a group of cancer patients undergoing radioimmunoglobulin therapy (RIT); SCE and acrolein-modified DNA were measured in cancer chemotherapy patients and in pharmacists preparing chemotherapy prescriptions; and SCE and PAH-DNA adducts are being measured in U.S. army troops stationed in Kuwait. Our results indicate that both SCE and PAH-DNA adduct levels were not elevated in firefighters, but that other factors such as smoking status and race were risk factors for increased SCE and PAH-DNA adducts. RIT was found to increase background rates of chromosome-type aberrations and frequencies of hprt mutations and there was a strong correlation between levels of therapy-induced chromosome damage sustained in vivo and in vitro sensitivity to radiation-induced chromosome damage. Peripheral blood lymphocytes of cancer patients treated with cyclophosphamide showed higher levels of SCE and had a higher incidence of acrolein adducts in DNA. Lymphocytes from pharmacists preparing antineoplastic drugs were found to acquire increased in vitro sensitivity to SCE induction by phosphoramide mustard with increased lifetime duration of drug handling. A prospective, longitudinal study was performed to identify environmental factors that modulate genetic damage in breast cancer patients. Women with benign breast masses and no apparent disease served as controls. Mutant frequency, cloning efficiency, and chromosomal aberration frequency did not differ significantly among the three groups. The results described and the data being gathered on troops stationed in Kuwait suggest that all the methodologies described can be useful in screening human populations for mutagenic exposures.

AB - Using a multidisciplinary approach, we have measured various indicators of DNA damage in peripheral lymphocytes of human populations potentially at increased risk for cancer. Sister chromatid exchanges (SCE) and polycyclic aromatic hydrocarbon (PAH)-DNA adducts were evaluated in a group of firefighters; chromosomal aberrations and hprt mutations were evaluated in a group of cancer patients undergoing radioimmunoglobulin therapy (RIT); SCE and acrolein-modified DNA were measured in cancer chemotherapy patients and in pharmacists preparing chemotherapy prescriptions; and SCE and PAH-DNA adducts are being measured in U.S. army troops stationed in Kuwait. Our results indicate that both SCE and PAH-DNA adduct levels were not elevated in firefighters, but that other factors such as smoking status and race were risk factors for increased SCE and PAH-DNA adducts. RIT was found to increase background rates of chromosome-type aberrations and frequencies of hprt mutations and there was a strong correlation between levels of therapy-induced chromosome damage sustained in vivo and in vitro sensitivity to radiation-induced chromosome damage. Peripheral blood lymphocytes of cancer patients treated with cyclophosphamide showed higher levels of SCE and had a higher incidence of acrolein adducts in DNA. Lymphocytes from pharmacists preparing antineoplastic drugs were found to acquire increased in vitro sensitivity to SCE induction by phosphoramide mustard with increased lifetime duration of drug handling. A prospective, longitudinal study was performed to identify environmental factors that modulate genetic damage in breast cancer patients. Women with benign breast masses and no apparent disease served as controls. Mutant frequency, cloning efficiency, and chromosomal aberration frequency did not differ significantly among the three groups. The results described and the data being gathered on troops stationed in Kuwait suggest that all the methodologies described can be useful in screening human populations for mutagenic exposures.

UR - http://www.scopus.com/inward/record.url?scp=0027741273&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027741273&partnerID=8YFLogxK

M3 - Article

C2 - 8143603

AN - SCOPUS:0027741273

VL - 101

SP - 121

EP - 125

JO - Environmental Health Perspectives

JF - Environmental Health Perspectives

SN - 0091-6765

IS - SUPPL. 3

ER -