Measurement of CD34+ cells in bone marrow by flow cytometry

T. M. Trischmann, K. G. Schepers, C. I. Civin

Research output: Contribution to journalArticle

Abstract

A procedure is described for the measurement of the %CD34+ progenitor cells in bone marrow using directly conjugated antibodies. Staining cells with anti-CD45.FITC in conjunction with anti-CD34.PE allows the CD45- nucleated red blood cells and the CD45++ lymphocytes and monocytes to be separated from the CD45+ progenitor cells. Granulocytes are separated from the CD34+ cells based on differences in side scatter properties. A gated acquisition of CD34+ cells is used to define the boundaries of the CD34+ population in a plot of forward scatter vs side scatter and in a plot of anti-CD45.FITC vs anti-CD34.PE. Use of these regions during analysis reduces background staining and allows for a consistent identification of a CD34+ population. Acquisition of 50,000 cells provides adequate precision of the %CD34+ measurement. Acquisition and analysis procedures are presented for use of both a Becton Dickinson FACScan flow cytometer and a Coulter EPICS Profile II flow cytometer.

Original languageEnglish (US)
Pages (from-to)305-313
Number of pages9
JournalJournal of Hematotherapy
Volume2
Issue number3
StatePublished - 1993

Fingerprint

Bone Marrow Cells
Flow Cytometry
Fluorescein-5-isothiocyanate
Stem Cells
Staining and Labeling
Granulocytes
Population
Monocytes
Erythrocytes
Bone Marrow
Lymphocytes
Antibodies

ASJC Scopus subject areas

  • Immunology
  • Hematology

Cite this

Trischmann, T. M., Schepers, K. G., & Civin, C. I. (1993). Measurement of CD34+ cells in bone marrow by flow cytometry. Journal of Hematotherapy, 2(3), 305-313.

Measurement of CD34+ cells in bone marrow by flow cytometry. / Trischmann, T. M.; Schepers, K. G.; Civin, C. I.

In: Journal of Hematotherapy, Vol. 2, No. 3, 1993, p. 305-313.

Research output: Contribution to journalArticle

Trischmann, TM, Schepers, KG & Civin, CI 1993, 'Measurement of CD34+ cells in bone marrow by flow cytometry', Journal of Hematotherapy, vol. 2, no. 3, pp. 305-313.
Trischmann TM, Schepers KG, Civin CI. Measurement of CD34+ cells in bone marrow by flow cytometry. Journal of Hematotherapy. 1993;2(3):305-313.
Trischmann, T. M. ; Schepers, K. G. ; Civin, C. I. / Measurement of CD34+ cells in bone marrow by flow cytometry. In: Journal of Hematotherapy. 1993 ; Vol. 2, No. 3. pp. 305-313.
@article{9e591af523824161a77307d5c341299c,
title = "Measurement of CD34+ cells in bone marrow by flow cytometry",
abstract = "A procedure is described for the measurement of the {\%}CD34+ progenitor cells in bone marrow using directly conjugated antibodies. Staining cells with anti-CD45.FITC in conjunction with anti-CD34.PE allows the CD45- nucleated red blood cells and the CD45++ lymphocytes and monocytes to be separated from the CD45+ progenitor cells. Granulocytes are separated from the CD34+ cells based on differences in side scatter properties. A gated acquisition of CD34+ cells is used to define the boundaries of the CD34+ population in a plot of forward scatter vs side scatter and in a plot of anti-CD45.FITC vs anti-CD34.PE. Use of these regions during analysis reduces background staining and allows for a consistent identification of a CD34+ population. Acquisition of 50,000 cells provides adequate precision of the {\%}CD34+ measurement. Acquisition and analysis procedures are presented for use of both a Becton Dickinson FACScan flow cytometer and a Coulter EPICS Profile II flow cytometer.",
author = "Trischmann, {T. M.} and Schepers, {K. G.} and Civin, {C. I.}",
year = "1993",
language = "English (US)",
volume = "2",
pages = "305--313",
journal = "Stem Cells and Development",
issn = "1547-3287",
publisher = "Mary Ann Liebert Inc.",
number = "3",

}

TY - JOUR

T1 - Measurement of CD34+ cells in bone marrow by flow cytometry

AU - Trischmann, T. M.

AU - Schepers, K. G.

AU - Civin, C. I.

PY - 1993

Y1 - 1993

N2 - A procedure is described for the measurement of the %CD34+ progenitor cells in bone marrow using directly conjugated antibodies. Staining cells with anti-CD45.FITC in conjunction with anti-CD34.PE allows the CD45- nucleated red blood cells and the CD45++ lymphocytes and monocytes to be separated from the CD45+ progenitor cells. Granulocytes are separated from the CD34+ cells based on differences in side scatter properties. A gated acquisition of CD34+ cells is used to define the boundaries of the CD34+ population in a plot of forward scatter vs side scatter and in a plot of anti-CD45.FITC vs anti-CD34.PE. Use of these regions during analysis reduces background staining and allows for a consistent identification of a CD34+ population. Acquisition of 50,000 cells provides adequate precision of the %CD34+ measurement. Acquisition and analysis procedures are presented for use of both a Becton Dickinson FACScan flow cytometer and a Coulter EPICS Profile II flow cytometer.

AB - A procedure is described for the measurement of the %CD34+ progenitor cells in bone marrow using directly conjugated antibodies. Staining cells with anti-CD45.FITC in conjunction with anti-CD34.PE allows the CD45- nucleated red blood cells and the CD45++ lymphocytes and monocytes to be separated from the CD45+ progenitor cells. Granulocytes are separated from the CD34+ cells based on differences in side scatter properties. A gated acquisition of CD34+ cells is used to define the boundaries of the CD34+ population in a plot of forward scatter vs side scatter and in a plot of anti-CD45.FITC vs anti-CD34.PE. Use of these regions during analysis reduces background staining and allows for a consistent identification of a CD34+ population. Acquisition of 50,000 cells provides adequate precision of the %CD34+ measurement. Acquisition and analysis procedures are presented for use of both a Becton Dickinson FACScan flow cytometer and a Coulter EPICS Profile II flow cytometer.

UR - http://www.scopus.com/inward/record.url?scp=0027818777&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027818777&partnerID=8YFLogxK

M3 - Article

C2 - 7522885

AN - SCOPUS:0027818777

VL - 2

SP - 305

EP - 313

JO - Stem Cells and Development

JF - Stem Cells and Development

SN - 1547-3287

IS - 3

ER -