Measurement of cardiac function using pressure-volume conductance catheter technique in mice and rats

Pál Pacher, Takahiro Nagayama, Partha Mukhopadhyay, Sándor Bátkai, David A Kass

Research output: Contribution to journalArticle

Abstract

Ventricular pressure-volume relationships have become well established as the most rigorous and comprehensive ways to assess intact heart function. Thanks to advances in miniature sensor technology, this approach has been successfully translated to small rodents, allowing for detailed characterization of cardiovascular function in genetically engineered mice, testing effects of pharmacotherapies and studying disease conditions. This method is unique for providing measures of left ventricular (LV) performance that are more specific to the heart and less affected by vascular loading conditions. Here we present descriptions and movies for procedures employing this method (anesthesia, intubation and surgical techniques, calibrations). We also provide examples of hemodynamics measurements obtained from normal mice/rats, and from animals with cardiac hypertrophy/heart failure, and describe values for various useful load-dependent and load-independent indexes of LV function obtained using different types of anesthesia. The completion of the protocol takes 1-4 h (depending on the experimental design/end points).

Original languageEnglish (US)
Pages (from-to)1422-1434
Number of pages13
JournalNature Protocols
Volume3
Issue number9
DOIs
StatePublished - 2008

Fingerprint

Catheters
Rats
Pressure
Anesthesia
Drug therapy
Cardiomegaly
Hemodynamics
Ventricular Pressure
Motion Pictures
Left Ventricular Function
Intubation
Design of experiments
Calibration
Blood Vessels
Rodentia
Animals
Research Design
Heart Failure
Technology
Drug Therapy

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Measurement of cardiac function using pressure-volume conductance catheter technique in mice and rats. / Pacher, Pál; Nagayama, Takahiro; Mukhopadhyay, Partha; Bátkai, Sándor; Kass, David A.

In: Nature Protocols, Vol. 3, No. 9, 2008, p. 1422-1434.

Research output: Contribution to journalArticle

Pacher, Pál ; Nagayama, Takahiro ; Mukhopadhyay, Partha ; Bátkai, Sándor ; Kass, David A. / Measurement of cardiac function using pressure-volume conductance catheter technique in mice and rats. In: Nature Protocols. 2008 ; Vol. 3, No. 9. pp. 1422-1434.
@article{05f5b4f97113408f97deebb476935b85,
title = "Measurement of cardiac function using pressure-volume conductance catheter technique in mice and rats",
abstract = "Ventricular pressure-volume relationships have become well established as the most rigorous and comprehensive ways to assess intact heart function. Thanks to advances in miniature sensor technology, this approach has been successfully translated to small rodents, allowing for detailed characterization of cardiovascular function in genetically engineered mice, testing effects of pharmacotherapies and studying disease conditions. This method is unique for providing measures of left ventricular (LV) performance that are more specific to the heart and less affected by vascular loading conditions. Here we present descriptions and movies for procedures employing this method (anesthesia, intubation and surgical techniques, calibrations). We also provide examples of hemodynamics measurements obtained from normal mice/rats, and from animals with cardiac hypertrophy/heart failure, and describe values for various useful load-dependent and load-independent indexes of LV function obtained using different types of anesthesia. The completion of the protocol takes 1-4 h (depending on the experimental design/end points).",
author = "P{\'a}l Pacher and Takahiro Nagayama and Partha Mukhopadhyay and S{\'a}ndor B{\'a}tkai and Kass, {David A}",
year = "2008",
doi = "10.1038/nprot.2008.138",
language = "English (US)",
volume = "3",
pages = "1422--1434",
journal = "Nature Protocols",
issn = "1754-2189",
publisher = "Nature Publishing Group",
number = "9",

}

TY - JOUR

T1 - Measurement of cardiac function using pressure-volume conductance catheter technique in mice and rats

AU - Pacher, Pál

AU - Nagayama, Takahiro

AU - Mukhopadhyay, Partha

AU - Bátkai, Sándor

AU - Kass, David A

PY - 2008

Y1 - 2008

N2 - Ventricular pressure-volume relationships have become well established as the most rigorous and comprehensive ways to assess intact heart function. Thanks to advances in miniature sensor technology, this approach has been successfully translated to small rodents, allowing for detailed characterization of cardiovascular function in genetically engineered mice, testing effects of pharmacotherapies and studying disease conditions. This method is unique for providing measures of left ventricular (LV) performance that are more specific to the heart and less affected by vascular loading conditions. Here we present descriptions and movies for procedures employing this method (anesthesia, intubation and surgical techniques, calibrations). We also provide examples of hemodynamics measurements obtained from normal mice/rats, and from animals with cardiac hypertrophy/heart failure, and describe values for various useful load-dependent and load-independent indexes of LV function obtained using different types of anesthesia. The completion of the protocol takes 1-4 h (depending on the experimental design/end points).

AB - Ventricular pressure-volume relationships have become well established as the most rigorous and comprehensive ways to assess intact heart function. Thanks to advances in miniature sensor technology, this approach has been successfully translated to small rodents, allowing for detailed characterization of cardiovascular function in genetically engineered mice, testing effects of pharmacotherapies and studying disease conditions. This method is unique for providing measures of left ventricular (LV) performance that are more specific to the heart and less affected by vascular loading conditions. Here we present descriptions and movies for procedures employing this method (anesthesia, intubation and surgical techniques, calibrations). We also provide examples of hemodynamics measurements obtained from normal mice/rats, and from animals with cardiac hypertrophy/heart failure, and describe values for various useful load-dependent and load-independent indexes of LV function obtained using different types of anesthesia. The completion of the protocol takes 1-4 h (depending on the experimental design/end points).

UR - http://www.scopus.com/inward/record.url?scp=51649116447&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=51649116447&partnerID=8YFLogxK

U2 - 10.1038/nprot.2008.138

DO - 10.1038/nprot.2008.138

M3 - Article

C2 - 18772869

AN - SCOPUS:51649116447

VL - 3

SP - 1422

EP - 1434

JO - Nature Protocols

JF - Nature Protocols

SN - 1754-2189

IS - 9

ER -