Abstract
The radioimmunoassay for the measurement of aldosterone in plasma (1) has been modified to permit measurement of pH 1 hydrolyzable aldosterone in urine and calculation of aldosterone secretion rate. Between 1/100 and 1/150 of a 24-hr urine specimen is necessary for the assay. Following CH2CI2 extraction, pH 1 hydrolysis is carried out for 24 hr. Purification of the aldosterone extract is achieved by 2 paper chromatographies. The technique can also be modified to permit the determination of urinary aldosterone excretion.
Original language | English (US) |
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Pages (from-to) | 507-510 |
Number of pages | 4 |
Journal | Journal of Clinical Endocrinology and Metabolism |
Volume | 31 |
Issue number | 5 |
DOIs | |
State | Published - 1970 |
Externally published | Yes |
ASJC Scopus subject areas
- Endocrinology, Diabetes and Metabolism
- Biochemistry
- Endocrinology
- Clinical Biochemistry
- Biochemistry, medical