Measurement of aldosterone secretion rate by radioimmunoassay

Francis Bayard, Inese Z. Beitins, Avinoam Kowarski, Claude J. Migeon

Research output: Contribution to journalArticlepeer-review


The radioimmunoassay for the measurement of aldosterone in plasma (1) has been modified to permit measurement of pH 1 hydrolyzable aldosterone in urine and calculation of aldosterone secretion rate. Between 1/100 and 1/150 of a 24-hr urine specimen is necessary for the assay. Following CH2CI2 extraction, pH 1 hydrolysis is carried out for 24 hr. Purification of the aldosterone extract is achieved by 2 paper chromatographies. The technique can also be modified to permit the determination of urinary aldosterone excretion.

Original languageEnglish (US)
Pages (from-to)507-510
Number of pages4
JournalJournal of Clinical Endocrinology and Metabolism
Issue number5
StatePublished - Jan 1 1970

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical


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