Measurement of aldosterone secretion rate by radioimmunoassay

Francis Bayard; Inese Z. Beitins; Avinoam Kowarski; Claude J. Migeon

doi:10.1210/jcem-31-5-507

Measurement of aldosterone secretion rate by radioimmunoassay

Francis Bayard, Inese Z. Beitins, Avinoam Kowarski, Claude J. Migeon

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

The radioimmunoassay for the measurement of aldosterone in plasma (1) has been modified to permit measurement of pH 1 hydrolyzable aldosterone in urine and calculation of aldosterone secretion rate. Between 1/100 and 1/150 of a 24-hr urine specimen is necessary for the assay. Following CH₂CI₂ extraction, pH 1 hydrolysis is carried out for 24 hr. Purification of the aldosterone extract is achieved by 2 paper chromatographies. The technique can also be modified to permit the determination of urinary aldosterone excretion.

Original language	English (US)
Pages (from-to)	507-510
Number of pages	4
Journal	Journal of Clinical Endocrinology and Metabolism
Volume	31
Issue number	5
DOIs	https://doi.org/10.1210/jcem-31-5-507
State	Published - 1970
Externally published	Yes

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Biochemistry
Endocrinology
Clinical Biochemistry
Biochemistry, medical

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author = "Francis Bayard and Beitins, {Inese Z.} and Avinoam Kowarski and Migeon, {Claude J.}",

year = "1970",

doi = "10.1210/jcem-31-5-507",

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T1 - Measurement of aldosterone secretion rate by radioimmunoassay

AU - Bayard, Francis

AU - Beitins, Inese Z.

AU - Kowarski, Avinoam

AU - Migeon, Claude J.

PY - 1970

Y1 - 1970

N2 - The radioimmunoassay for the measurement of aldosterone in plasma (1) has been modified to permit measurement of pH 1 hydrolyzable aldosterone in urine and calculation of aldosterone secretion rate. Between 1/100 and 1/150 of a 24-hr urine specimen is necessary for the assay. Following CH2CI2 extraction, pH 1 hydrolysis is carried out for 24 hr. Purification of the aldosterone extract is achieved by 2 paper chromatographies. The technique can also be modified to permit the determination of urinary aldosterone excretion.

AB - The radioimmunoassay for the measurement of aldosterone in plasma (1) has been modified to permit measurement of pH 1 hydrolyzable aldosterone in urine and calculation of aldosterone secretion rate. Between 1/100 and 1/150 of a 24-hr urine specimen is necessary for the assay. Following CH2CI2 extraction, pH 1 hydrolysis is carried out for 24 hr. Purification of the aldosterone extract is achieved by 2 paper chromatographies. The technique can also be modified to permit the determination of urinary aldosterone excretion.

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JO - Journal of Clinical Endocrinology and Metabolism

JF - Journal of Clinical Endocrinology and Metabolism

IS - 5

ER -

Measurement of aldosterone secretion rate by radioimmunoassay

Abstract

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