Measurement of aflatoxin and aflatoxin metabolites in urine by liquid chromatography-tandem mass spectrometry

Robert A. Everley; Frederic L. Ciner; Dongliang Zhang; Peter F. Scholl; John D. Groopman; Timothy R. Croley

doi:10.1093/jat/31.3.150

Measurement of aflatoxin and aflatoxin metabolites in urine by liquid chromatography-tandem mass spectrometry

Robert A. Everley, Frederic L. Ciner, Dongliang Zhang, Peter F. Scholl, John D. Groopman, Timothy R. Croley

Bloomberg School of Public Health

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Automated immunoaffinity solid-phase extraction followed by liquid chromatography-tandem mass spectrometry and chemical analogue internal standardization is employed to detect and quantify the aflatoxins AFB ₁, AFB₂, AFG₁, AFG₂, and the metabolites AFM₁ and AFP₁ in urine. The dynamic range of the method is nearly three orders of magnitude with limits of detection in the low femtogram on column range. The method was validated over a 12-day period by eight analysts. This method is suitable for agricultural, forensic, and public health laboratories during an accidental outbreak or a chemical terrorism event where a rapid and accurate diagnosis of aflatoxicosis is needed.

Original language	English (US)
Pages (from-to)	150-156
Number of pages	7
Journal	Journal of analytical toxicology
Volume	31
Issue number	3
DOIs	https://doi.org/10.1093/jat/31.3.150
State	Published - Apr 2007

ASJC Scopus subject areas

Analytical Chemistry
Environmental Chemistry
Toxicology
Health, Toxicology and Mutagenesis
Chemical Health and Safety

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10.1093/jat/31.3.150

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title = "Measurement of aflatoxin and aflatoxin metabolites in urine by liquid chromatography-tandem mass spectrometry",

abstract = "Automated immunoaffinity solid-phase extraction followed by liquid chromatography-tandem mass spectrometry and chemical analogue internal standardization is employed to detect and quantify the aflatoxins AFB 1, AFB2, AFG1, AFG2, and the metabolites AFM1 and AFP1 in urine. The dynamic range of the method is nearly three orders of magnitude with limits of detection in the low femtogram on column range. The method was validated over a 12-day period by eight analysts. This method is suitable for agricultural, forensic, and public health laboratories during an accidental outbreak or a chemical terrorism event where a rapid and accurate diagnosis of aflatoxicosis is needed.",

author = "Everley, {Robert A.} and Ciner, {Frederic L.} and Dongliang Zhang and Scholl, {Peter F.} and Groopman, {John D.} and Croley, {Timothy R.}",

note = "Funding Information: The authors would like to thank Erin Carson, Mike Martin, Chris Nixon, Jeff Snow, Shane Wyatt, and Jessica Zuckschw-erdt, who contributed to the validation process, and Janet Pruitt for the insightful discussions. The authors would also like to thank Dr. Torn York for his comments to the manuscript. Funding for the analytical work (R.E., EC., D.Z., and T.C.) was funded by the Centers for Disease Control and Prevention grant # U90/CCU317014. The animal study (P.S. and J.G.) work was funded by the NIH grants # P01 ES06052 and #P30 ES03819.",

year = "2007",

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doi = "10.1093/jat/31.3.150",

language = "English (US)",

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AU - Everley, Robert A.

AU - Ciner, Frederic L.

AU - Zhang, Dongliang

AU - Scholl, Peter F.

AU - Groopman, John D.

AU - Croley, Timothy R.

N1 - Funding Information: The authors would like to thank Erin Carson, Mike Martin, Chris Nixon, Jeff Snow, Shane Wyatt, and Jessica Zuckschw-erdt, who contributed to the validation process, and Janet Pruitt for the insightful discussions. The authors would also like to thank Dr. Torn York for his comments to the manuscript. Funding for the analytical work (R.E., EC., D.Z., and T.C.) was funded by the Centers for Disease Control and Prevention grant # U90/CCU317014. The animal study (P.S. and J.G.) work was funded by the NIH grants # P01 ES06052 and #P30 ES03819.

PY - 2007/4

Y1 - 2007/4

N2 - Automated immunoaffinity solid-phase extraction followed by liquid chromatography-tandem mass spectrometry and chemical analogue internal standardization is employed to detect and quantify the aflatoxins AFB 1, AFB2, AFG1, AFG2, and the metabolites AFM1 and AFP1 in urine. The dynamic range of the method is nearly three orders of magnitude with limits of detection in the low femtogram on column range. The method was validated over a 12-day period by eight analysts. This method is suitable for agricultural, forensic, and public health laboratories during an accidental outbreak or a chemical terrorism event where a rapid and accurate diagnosis of aflatoxicosis is needed.

AB - Automated immunoaffinity solid-phase extraction followed by liquid chromatography-tandem mass spectrometry and chemical analogue internal standardization is employed to detect and quantify the aflatoxins AFB 1, AFB2, AFG1, AFG2, and the metabolites AFM1 and AFP1 in urine. The dynamic range of the method is nearly three orders of magnitude with limits of detection in the low femtogram on column range. The method was validated over a 12-day period by eight analysts. This method is suitable for agricultural, forensic, and public health laboratories during an accidental outbreak or a chemical terrorism event where a rapid and accurate diagnosis of aflatoxicosis is needed.

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Measurement of aflatoxin and aflatoxin metabolites in urine by liquid chromatography-tandem mass spectrometry

Abstract

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