Measurement of aflatoxin and aflatoxin metabolites in urine by liquid chromatography-tandem mass spectrometry

Robert A. Everley, Frederic L. Ciner, Dongliang Zhang, Peter F. Scholl, John D. Groopman, Timothy R. Croley

Research output: Contribution to journalArticle

Abstract

Automated immunoaffinity solid-phase extraction followed by liquid chromatography-tandem mass spectrometry and chemical analogue internal standardization is employed to detect and quantify the aflatoxins AFB 1, AFB2, AFG1, AFG2, and the metabolites AFM1 and AFP1 in urine. The dynamic range of the method is nearly three orders of magnitude with limits of detection in the low femtogram on column range. The method was validated over a 12-day period by eight analysts. This method is suitable for agricultural, forensic, and public health laboratories during an accidental outbreak or a chemical terrorism event where a rapid and accurate diagnosis of aflatoxicosis is needed.

Original languageEnglish (US)
Pages (from-to)150-156
Number of pages7
JournalJournal of analytical toxicology
Volume31
Issue number3
DOIs
StatePublished - Apr 2007

ASJC Scopus subject areas

  • Analytical Chemistry
  • Environmental Chemistry
  • Toxicology
  • Health, Toxicology and Mutagenesis
  • Chemical Health and Safety

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