Measurement of aflatoxin and aflatoxin metabolites in urine by liquid chromatography-tandem mass spectrometry

Robert A. Everley, Frederic L. Ciner, Dongliang Zhang, Peter F. Scholl, John Davis Groopman, Timothy R. Croley

Research output: Contribution to journalArticle

Abstract

Automated immunoaffinity solid-phase extraction followed by liquid chromatography-tandem mass spectrometry and chemical analogue internal standardization is employed to detect and quantify the aflatoxins AFB 1, AFB2, AFG1, AFG2, and the metabolites AFM1 and AFP1 in urine. The dynamic range of the method is nearly three orders of magnitude with limits of detection in the low femtogram on column range. The method was validated over a 12-day period by eight analysts. This method is suitable for agricultural, forensic, and public health laboratories during an accidental outbreak or a chemical terrorism event where a rapid and accurate diagnosis of aflatoxicosis is needed.

Original languageEnglish (US)
Pages (from-to)150-156
Number of pages7
JournalJournal of Analytical Toxicology
Volume31
Issue number3
StatePublished - Apr 2007

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Aflatoxins
Terrorism
Liquid chromatography
Public health
Metabolites
Tandem Mass Spectrometry
Liquid Chromatography
urine
Standardization
Mass spectrometry
liquid chromatography
metabolite
mass spectrometry
Urine
Chemical Terrorism
terrorism
Solid Phase Extraction
standardization
Disease Outbreaks
Limit of Detection

ASJC Scopus subject areas

  • Analytical Chemistry
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Measurement of aflatoxin and aflatoxin metabolites in urine by liquid chromatography-tandem mass spectrometry. / Everley, Robert A.; Ciner, Frederic L.; Zhang, Dongliang; Scholl, Peter F.; Groopman, John Davis; Croley, Timothy R.

In: Journal of Analytical Toxicology, Vol. 31, No. 3, 04.2007, p. 150-156.

Research output: Contribution to journalArticle

Everley, Robert A. ; Ciner, Frederic L. ; Zhang, Dongliang ; Scholl, Peter F. ; Groopman, John Davis ; Croley, Timothy R. / Measurement of aflatoxin and aflatoxin metabolites in urine by liquid chromatography-tandem mass spectrometry. In: Journal of Analytical Toxicology. 2007 ; Vol. 31, No. 3. pp. 150-156.
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