Measurement of absolute amounts of antigen specific human IgE by a radioallergosorbent test (RAST) elution technique

R. R. Schellenberg, N Franklin Adkinson

Research output: Contribution to journalArticle

Abstract

A technique for the absolute quantification of antigen specific human IgE is described. It employs elution of a calculable amount of antigen specific IgE from an allergosorbent antibody complex by means of alkaline pH treatment, followed by measurement of the IgE content of the eluate with a modified radioimmunosirbent test (RIST).With this method IgE antibody directed against the benzylpencilloyl determinant of penicillin (BPO) was measured quantitatively in sera from seven penicillin allergic patients. IgE specific for ragweed antigen E was measured in sera from 33 ragweed allergic patients. Values obtained for IgE anti BPO ranged from 19 to 1806 ng/ml and comprised from 1.3 to 27.5% of total serum IgE. Values of IgE anti antigen E ranged from 9 to 1807 ng/ml, comprising from 3 to 84% of total serum IgE. Excellent correlation (r=0.99; p

Original languageEnglish (US)
Pages (from-to)1577-1583
Number of pages7
JournalJournal of Immunology
Volume115
Issue number6
StatePublished - 1975

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Radioallergosorbent Test
Immunoglobulin E
Antigens
Serum
Penicillins
Ambrosia
Antibodies

ASJC Scopus subject areas

  • Immunology

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Measurement of absolute amounts of antigen specific human IgE by a radioallergosorbent test (RAST) elution technique. / Schellenberg, R. R.; Adkinson, N Franklin.

In: Journal of Immunology, Vol. 115, No. 6, 1975, p. 1577-1583.

Research output: Contribution to journalArticle

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