MEASUREMENT OF 1,25‐DIHYDROXYCHOLECALCIFEROL IN MAN BY RADIOIMMUNOASSAY

T. L. CLEMENS, G. N. HENDY, S. E. PAPAPOULOS, L. J. FRAHER, A. D. CARE, J. L.H. O'RIORDAN

Research output: Contribution to journalArticle

Abstract

1α,25‐dihydroxycholecalciferol (1,25‐(OH)2D3) has been measured in human serum by radioimmunoassay. The assay uses a high titre antiserum raised in sheep against 1,25‐(OH)2D3‐25‐hemisuccinate, conjugated to bovine serum albumin. The sensitivity of the assay is 10 pg/tube. Other hydroxylated forms of vitamin D3 cross react with the antiserum and are therefore removed from serum extracts by chromatography on Sephadex LH 20 followed by high pressure liquid chromatography. The mean (± SEM) serum 1,25‐(OH)2D3 concentration for a group of healthy adult subjects was 41 ± 2.5 pg/ml. None was detected in anephric patients and the concentration was low or undetectable in patients with chronic renal failure.

Original languageEnglish (US)
Pages (from-to)225-234
Number of pages10
JournalClinical Endocrinology
Volume11
Issue number2
DOIs
StatePublished - Aug 1979
Externally publishedYes

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

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    CLEMENS, T. L., HENDY, G. N., PAPAPOULOS, S. E., FRAHER, L. J., CARE, A. D., & O'RIORDAN, J. L. H. (1979). MEASUREMENT OF 1,25‐DIHYDROXYCHOLECALCIFEROL IN MAN BY RADIOIMMUNOASSAY. Clinical Endocrinology, 11(2), 225-234. https://doi.org/10.1111/j.1365-2265.1979.tb03068.x