Measurement and partial characterization of an interleukin-2 inhibitor (IL-2-IN) in human urine

J. D. Fleischmann, S. M. Acino, K. M. Thomas, D. J. Riden, D. B. Wentworth, Z. Toossi, J. J. Ellner

Research output: Contribution to journalArticle

Abstract

We observed a human urine-derived protein complex (IL-2-IN) which competitively inhibits interleukin-2 (IL-2) dependent murine lymphocyte proliferation. Measurements of urinary IL-2-IN have been used to stratify the immmune response of patients to bacteria in the bladder. Partial characterization of IL-2-IN indicates that it is a heat-stable, 75 kDa complex comprised of interleukin-2 bound to another protein(s). Although the IL-2-IN complex is stable in physiologic buffers, the complex can be disrupted using acidic or low-ionic strength buffers, thereby liberating IL-2. IL-2-IN activity is susceptible to bacterial and endogenous urinary proteolysis. The IL-2 bound in the IL-2-IN complex cannot be detected using a double monoclonal antibody radioimmunoassay for IL-2. Unlike other IL-2 binding proteins, the IL-2 binding protein of the IL-2-IN complex is not a soluble interleukin-2 receptor. A modification of the bioassay for interleukin-2 activity is the method of choice for the detection and quantification of urinary IL-2-IN.

Original languageEnglish (US)
Pages (from-to)73-80
Number of pages8
JournalJournal of Biological Regulators and Homeostatic Agents
Volume4
Issue number2
StatePublished - 1990
Externally publishedYes

Fingerprint

interleukin-2
Interleukin-2
urine
Urine
interleukin 2 inhibitor
Carrier Proteins
Buffers
binding proteins
buffers
Interleukin-2 Receptors
lymphocyte proliferation
ionic strength
Biological Assay
radioimmunoassays
Osmolar Concentration
bladder
Proteolysis
Radioimmunoassay
proteolysis

ASJC Scopus subject areas

  • Immunology
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Physiology (medical)
  • Medicine (miscellaneous)
  • Physiology
  • Agricultural and Biological Sciences(all)

Cite this

Fleischmann, J. D., Acino, S. M., Thomas, K. M., Riden, D. J., Wentworth, D. B., Toossi, Z., & Ellner, J. J. (1990). Measurement and partial characterization of an interleukin-2 inhibitor (IL-2-IN) in human urine. Journal of Biological Regulators and Homeostatic Agents, 4(2), 73-80.

Measurement and partial characterization of an interleukin-2 inhibitor (IL-2-IN) in human urine. / Fleischmann, J. D.; Acino, S. M.; Thomas, K. M.; Riden, D. J.; Wentworth, D. B.; Toossi, Z.; Ellner, J. J.

In: Journal of Biological Regulators and Homeostatic Agents, Vol. 4, No. 2, 1990, p. 73-80.

Research output: Contribution to journalArticle

Fleischmann, JD, Acino, SM, Thomas, KM, Riden, DJ, Wentworth, DB, Toossi, Z & Ellner, JJ 1990, 'Measurement and partial characterization of an interleukin-2 inhibitor (IL-2-IN) in human urine', Journal of Biological Regulators and Homeostatic Agents, vol. 4, no. 2, pp. 73-80.
Fleischmann, J. D. ; Acino, S. M. ; Thomas, K. M. ; Riden, D. J. ; Wentworth, D. B. ; Toossi, Z. ; Ellner, J. J. / Measurement and partial characterization of an interleukin-2 inhibitor (IL-2-IN) in human urine. In: Journal of Biological Regulators and Homeostatic Agents. 1990 ; Vol. 4, No. 2. pp. 73-80.
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