Measles virus nucleocapsids: large-scale purification and use in radioimmunoassays

P. M E Moore, E. C. Hayes, S. E. Miller, L. L. Wright, Carolyn E Machamer, H. J. Zweerink

Research output: Contribution to journalArticle

Abstract

Nucleocapsids in quantities approaching 1 mg were purified from 109 measles virus-infected cells. They contained one polypeptide species with a molecular weight of 59,000. Antiserum was raised in rabbit against purified nucleocapsids and used in a competitive radioimmunoassay. Because of their instability, purified nucleocapsids were not suitable for use in such an assay. Instead, partially purified nucleocapsids from HEp-2 cells persistently infected with measles virus and labeled in vitro with [35S]methionine were used as the source of radioactive antigen. The radioimmunoassay thus developed measured less than 5 ng of nucleocapsids in infected cells.

Original languageEnglish (US)
Pages (from-to)842-846
Number of pages5
JournalInfection and Immunity
Volume20
Issue number3
StatePublished - 1978
Externally publishedYes

Fingerprint

Nucleocapsid
Measles virus
Radioimmunoassay
Methionine
Immune Sera
Molecular Weight
Rabbits
Antigens
Peptides

ASJC Scopus subject areas

  • Immunology

Cite this

Moore, P. M. E., Hayes, E. C., Miller, S. E., Wright, L. L., Machamer, C. E., & Zweerink, H. J. (1978). Measles virus nucleocapsids: large-scale purification and use in radioimmunoassays. Infection and Immunity, 20(3), 842-846.

Measles virus nucleocapsids : large-scale purification and use in radioimmunoassays. / Moore, P. M E; Hayes, E. C.; Miller, S. E.; Wright, L. L.; Machamer, Carolyn E; Zweerink, H. J.

In: Infection and Immunity, Vol. 20, No. 3, 1978, p. 842-846.

Research output: Contribution to journalArticle

Moore, PME, Hayes, EC, Miller, SE, Wright, LL, Machamer, CE & Zweerink, HJ 1978, 'Measles virus nucleocapsids: large-scale purification and use in radioimmunoassays', Infection and Immunity, vol. 20, no. 3, pp. 842-846.
Moore PME, Hayes EC, Miller SE, Wright LL, Machamer CE, Zweerink HJ. Measles virus nucleocapsids: large-scale purification and use in radioimmunoassays. Infection and Immunity. 1978;20(3):842-846.
Moore, P. M E ; Hayes, E. C. ; Miller, S. E. ; Wright, L. L. ; Machamer, Carolyn E ; Zweerink, H. J. / Measles virus nucleocapsids : large-scale purification and use in radioimmunoassays. In: Infection and Immunity. 1978 ; Vol. 20, No. 3. pp. 842-846.
@article{497f3582860d46628733341c5e5f02c5,
title = "Measles virus nucleocapsids: large-scale purification and use in radioimmunoassays",
abstract = "Nucleocapsids in quantities approaching 1 mg were purified from 109 measles virus-infected cells. They contained one polypeptide species with a molecular weight of 59,000. Antiserum was raised in rabbit against purified nucleocapsids and used in a competitive radioimmunoassay. Because of their instability, purified nucleocapsids were not suitable for use in such an assay. Instead, partially purified nucleocapsids from HEp-2 cells persistently infected with measles virus and labeled in vitro with [35S]methionine were used as the source of radioactive antigen. The radioimmunoassay thus developed measured less than 5 ng of nucleocapsids in infected cells.",
author = "Moore, {P. M E} and Hayes, {E. C.} and Miller, {S. E.} and Wright, {L. L.} and Machamer, {Carolyn E} and Zweerink, {H. J.}",
year = "1978",
language = "English (US)",
volume = "20",
pages = "842--846",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "3",

}

TY - JOUR

T1 - Measles virus nucleocapsids

T2 - large-scale purification and use in radioimmunoassays

AU - Moore, P. M E

AU - Hayes, E. C.

AU - Miller, S. E.

AU - Wright, L. L.

AU - Machamer, Carolyn E

AU - Zweerink, H. J.

PY - 1978

Y1 - 1978

N2 - Nucleocapsids in quantities approaching 1 mg were purified from 109 measles virus-infected cells. They contained one polypeptide species with a molecular weight of 59,000. Antiserum was raised in rabbit against purified nucleocapsids and used in a competitive radioimmunoassay. Because of their instability, purified nucleocapsids were not suitable for use in such an assay. Instead, partially purified nucleocapsids from HEp-2 cells persistently infected with measles virus and labeled in vitro with [35S]methionine were used as the source of radioactive antigen. The radioimmunoassay thus developed measured less than 5 ng of nucleocapsids in infected cells.

AB - Nucleocapsids in quantities approaching 1 mg were purified from 109 measles virus-infected cells. They contained one polypeptide species with a molecular weight of 59,000. Antiserum was raised in rabbit against purified nucleocapsids and used in a competitive radioimmunoassay. Because of their instability, purified nucleocapsids were not suitable for use in such an assay. Instead, partially purified nucleocapsids from HEp-2 cells persistently infected with measles virus and labeled in vitro with [35S]methionine were used as the source of radioactive antigen. The radioimmunoassay thus developed measured less than 5 ng of nucleocapsids in infected cells.

UR - http://www.scopus.com/inward/record.url?scp=0018185665&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018185665&partnerID=8YFLogxK

M3 - Article

C2 - 352962

AN - SCOPUS:0018185665

VL - 20

SP - 842

EP - 846

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 3

ER -