Measles virus nucleocapsids: large-scale purification and use in radioimmunoassays

Patricia M.E. Moore, Edward C. Hayes, Sara E. Miller, Larry L. Wright, Carolyn E. Machamer, Hans J. Zweerink

Research output: Contribution to journalArticlepeer-review

Abstract

Nucleocapsids in quantities approaching 1 mg were purified from 109 measles virus-infected cells. They contained one polypeptide species with a molecular weight of 59,000. Antiserum was raised in rabbit against purified nucleocapsids and used in a competitive radioimmunoassay. Because of their instability, purified nucleocapsids were not suitable for use in such an assay. Instead, partially purified nucleocapsids from HEp-2 cells persistently infected with measles virus and labeled in vitro with [35S]methionine were used as the source of radioactive antigen. The radioimmunoassay thus developed measured less than 5 ng of nucleocapsids in infected cells.

Original languageEnglish (US)
Pages (from-to)842-846
Number of pages5
JournalUnknown Journal
Volume20
Issue number3
DOIs
StatePublished - 1978

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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