MCM2/TOP2A (ProExC) immunohistochemistry as a predictive marker in head and neck mucosal biopsies

Erik G. Jenson, Michael Baker, Joseph A. Paydarfar, Benoit J. Gosselin, Zhigang Li, Candice C. Black

Research output: Contribution to journalArticle

Abstract

Mucosal biopsies from the head and neck are often small and poorly oriented, which impedes diagnostic interpretation, especially in patients with a history of cancer, being monitored for recurrence. A cocktail of antibodies targeted against DNA topoisomerase IIA and mini-chromosome maintenance protein 2 (MCM2/TOP2A, ProExC), markers of aberrant S-phase induction, have been used with success as a diagnostic adjunct in the evaluation of squamous dysplasia of the uterine cervix. We tested the utility in head and neck biopsies to see if ProExC could be used to discriminate reactive/inflammatory from true pre-neoplasia.Sixty-four archival biopsies were selected from patients who presented to the surgeon with an indication for biopsy to "rule out" dysplasia. Histologically, all biopsies showed nonspecific atypia that was difficult to discriminate from dysplasia. Twenty-three of the patients progressed to squamous carcinoma and the rest remained benign over five years follow-up. Cases stained with ProExC by IHC methods showed a significant pattern of expression (p=. 0.026). The staining was greatest in patients without a history of prior head and neck cancer but was not significant. Our results show that ProExC, used in conjunction with the H&E slide, can enhance the predictive power of a mucosal biopsy in a cohort of patients.

Original languageEnglish (US)
Pages (from-to)346-350
Number of pages5
JournalPathology Research and Practice
Volume210
Issue number6
DOIs
StatePublished - Jun 2014

Keywords

  • Biopsy interpretation
  • Dysplasia
  • Head and Neck
  • Immunohistochemisty
  • ProExC

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Cell Biology

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  • Cite this

    Jenson, E. G., Baker, M., Paydarfar, J. A., Gosselin, B. J., Li, Z., & Black, C. C. (2014). MCM2/TOP2A (ProExC) immunohistochemistry as a predictive marker in head and neck mucosal biopsies. Pathology Research and Practice, 210(6), 346-350. https://doi.org/10.1016/j.prp.2014.02.003