Maximum likelihood 3-D reconstruction of multiple viruses from mixtures of cryo electron microscope images

Junghoon Lee, Yili Zheng, Peter C. Doerschuk, Jinghua Tang, John E. Johnson

Research output: Contribution to journalConference articlepeer-review

Abstract

3-D reconstruction of the electron scattering intensity of a virus from cryo electron microscopy is essentially a 3-D tomography problem in which the orientation of the 2-D projections is unknown. Many biological problems concern mixtures of different types of virus particles or mixtures of different maturation states of the same type of virus particle. For a variety of reasons, especially low SNR, it can be very challenging to label the type or state shown in an individual image. Algorithms capable of computing multiple reconstructions, one for each type or state, based on images which are not labeled according to type or state, are described and demonstrated on experimental images.

Original languageEnglish (US)
Article number34
Pages (from-to)336-343
Number of pages8
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume5674
DOIs
StatePublished - Jul 20 2005
Externally publishedYes
EventProceedings of SPIE-IS and T Electronic Imaging - Computational Imaging III - San Jose, CA, United States
Duration: Jan 17 2005Jan 18 2005

Keywords

  • 3-D reconstruction
  • Statistical reconstruction
  • Tomography
  • Virus structure

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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