Matrix‐assisted laser desorption of large biomolecules with a TEA‐CO2‐laser

A. Overberg; M. Karas; F. Hillenkamp; R. J. Cotter

doi:10.1002/rcm.1290050308

Matrix‐assisted laser desorption of large biomolecules with a TEA‐CO₂‐laser

A. Overberg, M. Karas, F. Hillenkamp, R. J. Cotter

Research output: Contribution to journal › Article › peer-review

118 Scopus citations

Abstract

Matrix‐assisted desorption/ionization of large biomolecules with a TEA‐CO₂ laser with a warelength of 10.6 μm is demonstrated. TEA‐CO₂ LDI mass spectra are almost identical to those obtained with the Er‐YAG (2.94 μm) infra‐red (IR) laser and very similar to UV‐LDI spectra. Most of the matrices usable for Er‐YAG IR‐LDI have been tested at 10.6 μm with success, and the main features of IR‐LDI e.g., the tendency towards the formation of higher chargers is shown.

Original language	English (US)
Pages (from-to)	128-131
Number of pages	4
Journal	Rapid Communications in Mass Spectrometry
Volume	5
Issue number	3
DOIs	https://doi.org/10.1002/rcm.1290050308
State	Published - Mar 1991
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry
Spectroscopy
Organic Chemistry

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10.1002/rcm.1290050308

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abstract = "Matrix‐assisted desorption/ionization of large biomolecules with a TEA‐CO2 laser with a warelength of 10.6 μm is demonstrated. TEA‐CO2 LDI mass spectra are almost identical to those obtained with the Er‐YAG (2.94 μm) infra‐red (IR) laser and very similar to UV‐LDI spectra. Most of the matrices usable for Er‐YAG IR‐LDI have been tested at 10.6 μm with success, and the main features of IR‐LDI e.g., the tendency towards the formation of higher chargers is shown.",

author = "A. Overberg and M. Karas and F. Hillenkamp and Cotter, {R. J.}",

year = "1991",

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T1 - Matrix‐assisted laser desorption of large biomolecules with a TEA‐CO2‐laser

AU - Overberg, A.

AU - Karas, M.

AU - Hillenkamp, F.

AU - Cotter, R. J.

PY - 1991/3

Y1 - 1991/3

N2 - Matrix‐assisted desorption/ionization of large biomolecules with a TEA‐CO2 laser with a warelength of 10.6 μm is demonstrated. TEA‐CO2 LDI mass spectra are almost identical to those obtained with the Er‐YAG (2.94 μm) infra‐red (IR) laser and very similar to UV‐LDI spectra. Most of the matrices usable for Er‐YAG IR‐LDI have been tested at 10.6 μm with success, and the main features of IR‐LDI e.g., the tendency towards the formation of higher chargers is shown.

AB - Matrix‐assisted desorption/ionization of large biomolecules with a TEA‐CO2 laser with a warelength of 10.6 μm is demonstrated. TEA‐CO2 LDI mass spectra are almost identical to those obtained with the Er‐YAG (2.94 μm) infra‐red (IR) laser and very similar to UV‐LDI spectra. Most of the matrices usable for Er‐YAG IR‐LDI have been tested at 10.6 μm with success, and the main features of IR‐LDI e.g., the tendency towards the formation of higher chargers is shown.

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ER -

Matrix‐assisted laser desorption of large biomolecules with a TEA‐CO₂‐laser

Abstract

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